Universität zu Köln

Brischigliaro, Michele ORCID: 0000-0003-1520-1342, Cabrera-Orefice, Alfredo ORCID: 0000-0002-2042-3794, Sturlese, Mattia, Elurbe, Dei M., Frigo, Elena, Fernandez-Vizarra, Erika ORCID: 0000-0002-2469-142X, Moro, Stefano, Huynen, Martijn A., Arnold, Susanne ORCID: 0000-0003-2667-5156, Viscomi, Carlo and Zeviani, Massimo (2022). CG7630 is the Drosophila melanogaster homolog of the cytochrome c oxidase subunit COX7B. EMBO Rep., 23 (8). HOBOKEN: WILEY. ISSN 1469-3178

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Abstract

The mitochondrial respiratory chain (MRC) is composed of four multiheteromeric enzyme complexes. According to the endosymbiotic origin of mitochondria, eukaryotic MRC derives from ancestral proteobacterial respiratory structures consisting of a minimal set of complexes formed by a few subunits associated with redox prosthetic groups. These enzymes, which are the core redox centers of respiration, acquired additional subunits, and increased their complexity throughout evolution. Cytochrome c oxidase (COX), the terminal component of MRC, has a highly interspecific heterogeneous composition. Mammalian COX consists of 14 different polypeptides, of which COX7B is considered the evolutionarily youngest subunit. We applied proteomic, biochemical, and genetic approaches to investigate the COX composition in the invertebrate model Drosophila melanogaster. We identified and characterized a novel subunit which is widely different in amino acid sequence, but similar in secondary and tertiary structures to COX7B, and provided evidence that this object is in fact replacing the latter subunit in virtually all protostome invertebrates. These results demonstrate that although individual structures may differ the composition of COX is functionally conserved between vertebrate and invertebrate species.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Brischigliaro, Michele UNSPECIFIED orcid.org/0000-0003-1520-1342 UNSPECIFIED Cabrera-Orefice, Alfredo UNSPECIFIED orcid.org/0000-0002-2042-3794 UNSPECIFIED Sturlese, Mattia UNSPECIFIED UNSPECIFIED UNSPECIFIED Elurbe, Dei M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Frigo, Elena UNSPECIFIED UNSPECIFIED UNSPECIFIED Fernandez-Vizarra, Erika UNSPECIFIED orcid.org/0000-0002-2469-142X UNSPECIFIED Moro, Stefano UNSPECIFIED UNSPECIFIED UNSPECIFIED Huynen, Martijn A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Arnold, Susanne UNSPECIFIED orcid.org/0000-0003-2667-5156 UNSPECIFIED Viscomi, Carlo UNSPECIFIED UNSPECIFIED UNSPECIFIED Zeviani, Massimo UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-677765
DOI:	10.15252/embr.202254825
Journal or Publication Title:	EMBO Rep.
Volume:	23
Number:	8
Date:	2022
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1469-3178
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MITOCHONDRIAL COMPLEX; PREDICTION; MUTATIONS; EVOLUTION; ACCURACY; NDUFA4; CHAIN Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67776