Universität zu Köln

Masangkay, Frederick R., Milanez, Giovanni D., Dionisio, Joseph D., Ormita, Luzelle Anne G. -L., V. Alvarez, Abel and Karanis, Panagiotis (2022). Well water sources simultaneous contamination with Cryptosporidium and Acanthamoeba in East-Southeast Asia and Acanthamoeba spp. in biofilms in the Philippines. Sci. Total Environ., 837. AMSTERDAM: ELSEVIER. ISSN 1879-1026

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Abstract

Cryptosporidium is the leading agent of waterborne parasitic protozoan outbreaks and is the second leading cause of infant mortality due to diarrhoea worldwide. Acanthamoeba spp. causes Acanthamoeba keratitis (AK) and a life threatening condition known as granulomatous amoebic encephalitis (GAE). The present study aimed to assess the water quality of an indigenous and a rural community for waterborne parasitic protozoan contamination. Aquatic samples (n = 22) were processed by filtration of 500 mL portion through a 1.2 mu m pore size glass microfiber filter and eluted for light microscopy, culture in non-nutrient agar, and PCR analysis. Overall, 36% (8/22) of the investigated aquatic samples were positive for either Cryptosporidium spp. oocysts (13%; 3/22) or Acanthamoeba spp., (36%; 8/ 22) or both (13%; 3/22). Cryptosporidium spp. oocysts were detected in 27% (3/11) of wet season samples only while Acanthamoeba spp. were detected in 18% (2/11) and 55% (6/11) of wet and dry season samples, respectively. Subsequently, molecular detection for Acanthamoeba species identified A. lenticulata and A. hatchetti with 98-99% BLAST similarity. This is the first report on the simultaneous contamination of Cryptosporidium and Acanthamoeba in well water sources in East-Southeast Asia, the first detection of Acanthamoeba spp. in biofilms in the Philippines, and the longest viability demonstrated for A. lenticulata in two-year-old water samples stored at room temperature.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Masangkay, Frederick R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Milanez, Giovanni D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dionisio, Joseph D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ormita, Luzelle Anne G. -L. UNSPECIFIED UNSPECIFIED UNSPECIFIED V. Alvarez, Abel UNSPECIFIED UNSPECIFIED UNSPECIFIED Karanis, Panagiotis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-677914
DOI:	10.1016/j.scitotenv.2022.155752
Journal or Publication Title:	Sci. Total Environ.
Volume:	837
Date:	2022
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1879-1026
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FREE-LIVING AMEBAS; PROTOZOAN PARASITES; MOLECULAR CHARACTERIZATION; WORLDWIDE OUTBREAKS; PARVUM OOCYSTS; TAP WATER; IDENTIFICATION; TRANSMISSION; INFECTION; GENOTYPES Multiple languages Environmental Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67791