Universität zu Köln

Erdek, Oezlem, Bloch, Wilhelm, Rink-Notzon, Svenja, Roggendorf, Hubert C., Uzun, Senem, Meul, Britta, Koch, Manuel ORCID: 0000-0002-2962-7814, Neugebauer, Joerg, Deschner, James and Korkmaz, Yueksel (2022). Inflammation of the Human Dental Pulp Induces Phosphorylation of eNOS at Thr495 in Blood Vessels. Biomedicines, 10 (7). BASEL: MDPI. ISSN 2227-9059

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Abstract

The activity of endothelial nitric oxide synthase (eNOS) in endothelial cells increased with the phosphorylation of the enzyme at Ser1177 and decreased at Thr495. The regulation of the phosphorylation sites of eNOS at Ser1177 and Thr495 in blood vessels of the healthy and inflamed human dental pulp is unknown. To investigate this, healthy and carious human third molars were immersion-fixed and decalcified. The localization of eNOS, Ser1177, and Thr495 in healthy and inflamed blood vessels was examined in consecutive cryo-sections using quantitative immunohistochemical methods. We found that the staining intensity of Ser1177 in healthy blood vessels decreased in inflamed blood vessels, whereas the weak staining intensity of Thr495 in healthy blood vessels strongly increased in inflamed blood vessels. In blood vessels of the healthy pulp, eNOS is active with phosphorylation of the enzyme at Ser1177. The phosphorylation of eNOS at Thr495 in inflamed blood vessels leads to a decrease in eNOS activity, contributing to eNOS uncoupling and giving evidence for a decrease in NO and an increase in O-2(-) production. Since the formation of the tertiary dentin matrix depends on intact pulp circulation, eNOS uncoupling and phosphorylation of eNOS at Thr495 in the inflamed pulp blood vessels should be considered during caries therapy.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Erdek, Oezlem UNSPECIFIED UNSPECIFIED UNSPECIFIED Bloch, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Rink-Notzon, Svenja UNSPECIFIED UNSPECIFIED UNSPECIFIED Roggendorf, Hubert C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Uzun, Senem UNSPECIFIED UNSPECIFIED UNSPECIFIED Meul, Britta UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Manuel UNSPECIFIED orcid.org/0000-0002-2962-7814 UNSPECIFIED Neugebauer, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED Deschner, James UNSPECIFIED UNSPECIFIED UNSPECIFIED Korkmaz, Yueksel UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-678015
DOI:	10.3390/biomedicines10071586
Journal or Publication Title:	Biomedicines
Volume:	10
Number:	7
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2227-9059
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NITRIC-OXIDE SYNTHASE; INTERSTITIAL FLUID PRESSURE; SOLUBLE GUANYLATE-CYCLASE; ENDOTHELIAL NO SYNTHASE; OXIDATIVE STRESS; MOLECULAR-MECHANISMS; THERAPEUTIC TARGET; VASCULAR-DISEASE; PROTEIN-KINASE; T-CELL Multiple languages Biochemistry & Molecular Biology; Medicine, Research & Experimental; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67801