Universität zu Köln

Jaudal, Mauren ORCID: 0000-0002-5760-2497, Mayo-Smith, Matthew, Poulet, Axel, Whibley, Annabel, Peng, Yongyan ORCID: 0000-0002-6392-8119, Zhang, Lulu, Thomson, Geoffrey, Trimborn, Laura, Jacob, Yannick, van Wolfswinkel, Josien C., Goldstone, David C., Wen, Jiangqi ORCID: 0000-0001-5113-7750, Mysore, Kirankumar S. and Putterill, Joanna (2022). MtING2 encodes an ING domain PHD finger protein which affects Medicago growth, flowering, global patterns of H3K4me3, and gene expression. Plant J., 112 (4). S. 1029 - 1051. HOBOKEN: WILEY. ISSN 1365-313X

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Abstract

Flowering of the reference legume Medicago truncatula is promoted by winter cold (vernalization) followed by long-day photoperiods (VLD) similar to winter annual Arabidopsis. However, Medicago lacks FLC and CO, key regulators of Arabidopsis VLD flowering. Most plants have two INHIBITOR OF GROWTH (ING) genes (ING1 and ING2), encoding proteins with an ING domain with two anti-parallel alpha-helices and a plant homeodomain (PHD) finger, but their genetic role has not been previously described. In Medicago, Mting1 gene-edited mutants developed and flowered normally, but an Mting2-1 Tnt1 insertion mutant and gene-edited Mting2 mutants had developmental abnormalities including delayed flowering particularly in VLD, compact architecture, abnormal leaves with extra leaflets but no trichomes, and smaller seeds and barrels. Mting2 mutants had reduced expression of activators of flowering, including the FT-like gene MtFTa1, and increased expression of the candidate repressor MtTFL1c, consistent with the delayed flowering of the mutant. MtING2 overexpression complemented Mting2-1, but did not accelerate flowering in wild type. The MtING2 PHD finger bound H3K4me2/3 peptides weakly in vitro, but analysis of gene-edited mutants indicated that it was dispensable to MtING2 function in wild-type plants. RNA sequencing experiments indicated that >7000 genes are mis-expressed in the Mting2-1 mutant, consistent with its strong mutant phenotypes. Interestingly, ChIP-seq analysis identified >5000 novel H3K4me3 locations in the genome of Mting2-1 mutants compared to wild type R108. Overall, our mutant study has uncovered an important physiological role of a plant ING2 gene in development, flowering, and gene expression, which likely involves an epigenetic mechanism.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Jaudal, Mauren UNSPECIFIED orcid.org/0000-0002-5760-2497 UNSPECIFIED Mayo-Smith, Matthew UNSPECIFIED UNSPECIFIED UNSPECIFIED Poulet, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED Whibley, Annabel UNSPECIFIED UNSPECIFIED UNSPECIFIED Peng, Yongyan UNSPECIFIED orcid.org/0000-0002-6392-8119 UNSPECIFIED Zhang, Lulu UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomson, Geoffrey UNSPECIFIED UNSPECIFIED UNSPECIFIED Trimborn, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED Jacob, Yannick UNSPECIFIED UNSPECIFIED UNSPECIFIED van Wolfswinkel, Josien C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Goldstone, David C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wen, Jiangqi UNSPECIFIED orcid.org/0000-0001-5113-7750 UNSPECIFIED Mysore, Kirankumar S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Putterill, Joanna UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-679945
DOI:	10.1111/tpj.15994
Journal or Publication Title:	Plant J.
Volume:	112
Number:	4
Page Range:	S. 1029 - 1051
Date:	2022
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-313X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ARABIDOPSIS-THALIANA; PLANT HOMEODOMAIN; VERNALIZATION; TRUNCATULA; FAMILY; LOCUS; TIME; TRANSFORMATION; MUTAGENESIS; ORGANIZATION Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67994