Universität zu Köln

Biehl, Lena M. ORCID: 0000-0001-7613-8119, Farowski, Fedja, Hilpert, Catharina, Nowag, Angela, Kretzschmar, Anne, Jazmati, Nathalie, Tsakmaklis, Anastasia, Wieters, Imke, Khodamoradi, Yascha ORCID: 0000-0001-5972-0967, Wisplinghoff, Hilmar and Vehreschild, Maria J. G. T. (2022). Longitudinal variability in the urinary microbiota of healthy premenopausal women and the relation to neighboring microbial communities: A pilot study. PLoS One, 17 (1). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Background The understanding of longitudinal changes in the urinary microbiota of healthy women and its relation to intestinal microbiota is limited. Methods From a cohort of 15 premenopausal women without known urogenital disease or current symptoms, we collected catheter urine (CU), vaginal and periurethral swabs, and fecal samples on four visits over six months. Additionally, ten participants provided CU and midstream urine (MU) to assess comparability. Urine was subjected to expanded culture. 16S rRNA gene sequencing was performed on all urine, fecal, and selected vaginal and periurethral samples. Sequence reads were processed (DADA2 pipeline) and analyzed using QIIME 2 and R. Results Relative abundances of urinary microbiota were variable over 6-18 months. The degree of intraindividual variability of urinary microbiota was higher than that found in fecal samples. Still, nearly half of the observed beta diversity of all urine samples could be attributed to differences between volunteers (R-2 = 0.48, p = 0.001). After stratification by volunteer, time since last sexual intercourse was shown to be a factor significantly contributing to beta diversity (R-2 = 0.14, p = 0.001). We observed a close relatedness of urogenital microbial habitats and a clear distinction from intestinal microbiota in the overall betadiversity analysis. Microbiota compositions derived from MU differed only slightly from CU compositions. Within this analysis of low-biomass samples, we identified contaminating sequences potentially stemming from sequencing reagents. Conclusions Results from our longitudinal cohort study confirmed the presence of a rather variable individual urinary microbiota in premenopausal women. These findings from catheter urine complement previous observations on temporal dynamics in voided urine. The higher intraindividual variability of urinary microbiota as compared to fecal microbiota will be a challenge for future studies investigating associations with urogenital diseases and aiming at identifying pathogenic microbiota signatures.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Biehl, Lena M. UNSPECIFIED orcid.org/0000-0001-7613-8119 UNSPECIFIED Farowski, Fedja UNSPECIFIED UNSPECIFIED UNSPECIFIED Hilpert, Catharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Nowag, Angela UNSPECIFIED UNSPECIFIED UNSPECIFIED Kretzschmar, Anne UNSPECIFIED UNSPECIFIED UNSPECIFIED Jazmati, Nathalie UNSPECIFIED UNSPECIFIED UNSPECIFIED Tsakmaklis, Anastasia UNSPECIFIED UNSPECIFIED UNSPECIFIED Wieters, Imke UNSPECIFIED UNSPECIFIED UNSPECIFIED Khodamoradi, Yascha UNSPECIFIED orcid.org/0000-0001-5972-0967 UNSPECIFIED Wisplinghoff, Hilmar UNSPECIFIED UNSPECIFIED UNSPECIFIED Vehreschild, Maria J. G. T. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-680032
DOI:	10.1371/journal.pone.0262095
Journal or Publication Title:	PLoS One
Volume:	17
Number:	1
Date:	2022
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BACTERIA; CULTURE Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68003