Universität zu Köln

Yu, Dongli, Song, Wen ORCID: 0000-0002-9498-2409, Tan, Eddie Yong Jun, Liu, Li, Cao, Yu, Jirschitzka, Jan, Li, Ertong, Logemann, Elke ORCID: 0000-0002-0478-8072, Xu, Chenrui, Huang, Shijia, Jia, Aolin, Chang, Xiaoyu, Han, Zhifu, Wu, Bin, Schulze-Lefert, Paul and Chai, Jijie (2022). TIR domains of plant immune receptors are 2 ',3 '-cAMP/cGMP synthetases mediating cell death. Cell, 185 (13). S. 2370 - 2406. CAMBRIDGE: CELL PRESS. ISSN 1097-4172

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Abstract

2',3'-cAMP is a positional isomer of the well-established second messenger 3',5'-cAMP, but little is known about the biology of this noncanonical cyclic nucleotide monophosphate (cNMP). Toll/interleukin-1 receptor (TIR) domains of nucleotide-binding leucine-rich repeat (NLR) immune receptors have the NADase function necessary but insufficient to activate plant immune responses. Here, we show that plant TIR proteins, besides being NADases, act as 2',3'-cAMP/cGMP synthetases by hydrolyzing RNA/DNA. Structural data show that a TIR domain adopts distinct oligomers with mutually exclusive NADase and synthetase activity. Mutations specifically disrupting the synthetase activity abrogate TIR-mediated cell death in Nicotiana benthamiana (Nb), supporting an important role for these cNMPs in TIR signaling. Furthermore, the Arabidopsis negative regulator of TIR-NLR signaling, NUDT7, displays 2',3'-cAMP/cGMP but not 3',5'-cAMP/cGMP phosphodiesterase activity and suppresses cell death activity of TIRs in Nb. Our study identifies a family of 2',3'-cAMP/cGMP synthetases and establishes a critical role for them in plant immune responses.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Yu, Dongli UNSPECIFIED UNSPECIFIED UNSPECIFIED Song, Wen UNSPECIFIED orcid.org/0000-0002-9498-2409 UNSPECIFIED Tan, Eddie Yong Jun UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Li UNSPECIFIED UNSPECIFIED UNSPECIFIED Cao, Yu UNSPECIFIED UNSPECIFIED UNSPECIFIED Jirschitzka, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Li, Ertong UNSPECIFIED UNSPECIFIED UNSPECIFIED Logemann, Elke UNSPECIFIED orcid.org/0000-0002-0478-8072 UNSPECIFIED Xu, Chenrui UNSPECIFIED UNSPECIFIED UNSPECIFIED Huang, Shijia UNSPECIFIED UNSPECIFIED UNSPECIFIED Jia, Aolin UNSPECIFIED UNSPECIFIED UNSPECIFIED Chang, Xiaoyu UNSPECIFIED UNSPECIFIED UNSPECIFIED Han, Zhifu UNSPECIFIED UNSPECIFIED UNSPECIFIED Wu, Bin UNSPECIFIED UNSPECIFIED UNSPECIFIED Schulze-Lefert, Paul UNSPECIFIED UNSPECIFIED UNSPECIFIED Chai, Jijie UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-680213
DOI:	10.1016/j.cell.2022.04.032
Journal or Publication Title:	Cell
Volume:	185
Number:	13
Page Range:	S. 2370 - 2406
Date:	2022
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1097-4172
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language 2',3'-CYCLIC NUCLEOTIDE 3'-PHOSPHODIESTERASE; PATHOGEN EFFECTOR; CYCLIC GUANOSINE; SELF-ASSOCIATION; STRUCTURAL BASIS; RNASE T2; ARABIDOPSIS; PROTEIN; RESISTANCE; RESISTOSOME Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68021