Universität zu Köln

Feles, Sebastian ORCID: 0000-0002-5008-6290, Overath, Christian, Reichardt, Sina, Diegeler, Sebastian ORCID: 0000-0003-3161-0744, Schmitz, Claudia, Kronenberg, Jessica, Baumstark-Khan, Christa, Hemmersbach, Ruth, Hellweg, Christine E. and Liemersdorf, Christian ORCID: 0000-0001-8407-5226 (2022). Streamlining Culture Conditions for the Neuroblastoma Cell Line SH-SY5Y: A Prerequisite for Functional Studies. Methods Protoc., 5 (4). BASEL: MDPI. ISSN 2409-9279

Full text not available from this repository.

Abstract

The neuroblastoma cell line SH-SY5Y has been a well-established and very popular in vitro model in neuroscience for decades, especially focusing on neurodevelopmental disorders, such as Parkinson's disease. The ability of this cell type to differentiate compared with other models in neurobiology makes it one of the few suitable models without having to rely on a primary culture of neuronal cells. Over the years, various, partly contradictory, methods of cultivation have been reported. This study is intended to provide a comprehensive guide to the in vitro cultivation of undifferentiated SH-SY5Y cells. For this purpose, the morphology of the cell line and the differentiation of the individual subtypes are described, and instructions for cell culture practice and long-term cryoconservation are provided. We describe the key growth characteristics of this cell line, including proliferation and confluency data, optimal initial seeding cell numbers, and a comparison of different culture media and cell viability during cultivation. Furthermore, applying an optimized protocol in a long-term cultivation over 60 days, we show that cumulative population doubling (CPD) is constant over time and does not decrease with incremental passage, enabling stable cultivation, for example, for recurrent differentiation to achieve the highest possible reproducibility in subsequent analyses. Therefore, we provide a solid guidance for future research that employs the neuroblastoma cell line SH-SY5Y.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Feles, Sebastian UNSPECIFIED orcid.org/0000-0002-5008-6290 UNSPECIFIED Overath, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Reichardt, Sina UNSPECIFIED UNSPECIFIED UNSPECIFIED Diegeler, Sebastian UNSPECIFIED orcid.org/0000-0003-3161-0744 UNSPECIFIED Schmitz, Claudia UNSPECIFIED UNSPECIFIED UNSPECIFIED Kronenberg, Jessica UNSPECIFIED UNSPECIFIED UNSPECIFIED Baumstark-Khan, Christa UNSPECIFIED UNSPECIFIED UNSPECIFIED Hemmersbach, Ruth UNSPECIFIED UNSPECIFIED UNSPECIFIED Hellweg, Christine E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Liemersdorf, Christian UNSPECIFIED orcid.org/0000-0001-8407-5226 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-681075
DOI:	10.3390/mps5040058
Journal or Publication Title:	Methods Protoc.
Volume:	5
Number:	4
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2409-9279
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IN-VITRO MODEL; DIFFERENTIATED SH-SY5Y; NEURITE OUTGROWTH; RETINOIC ACID; EXPRESSION; GROWTH; ERYTHROPOIETIN; RECEPTOR Multiple languages Biochemical Research Methods Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68107