Jia, Aolin, Huang, Shijia, Song, Wen ORCID: 0000-0002-9498-2409, Wang, Junli ORCID: 0000-0001-8825-3894, Meng, Yonggang, Sun, Yue, Xu, Lina, Laessle, Henriette, Jirschitzka, Jan ORCID: 0000-0003-1007-4700, Hou, Jiao ORCID: 0000-0002-2945-1791, Zhang, Tiantian, Yu, Wenquan ORCID: 0000-0002-3711-0006, Hessler, Giuliana, Li, Ertong, Ma, Shoucai, Yu, Dongli, Gebauer, Jan ORCID: 0000-0002-3989-4748, Baumann, Ulrich, Liu, Xiaohui, Han, Zhifu, Chang, Junbiao, Parker, Jane E. and Chai, Jijie (2022). TIR-catalyzed ADP-ribosylation reactions produce signaling molecules for plant immunity. Science, 377 (6605). S. 488 - 497. WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE. ISSN 1095-9203

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Abstract

Plant pathogen-activated immune signaling by nucleotide-binding leucine-rich repeat (NLR) receptors with an N-terminal Toll/interleukin-1 receptor (TIR) domain converges on Enhanced Disease Susceptibility 1 (EDS1) and its direct partners, Phytoalexin Deficient 4 (PAD4) or Senescence-Associated Gene 101 (SAG101). TIR-encoded nicotinamide adenine dinucleotide hydrolase (NADase) produces signaling molecules to promote exclusive EDS1-PAD4 and EDS1-SAG101 interactions with helper NLR subclasses. In this work, we show that TIR-containing proteins catalyze adenosine diphosphate (ADP)-ribosylation of adenosine triphosphate (ATP) and ADP ribose (ADPR) through ADPR polymeraselike and NADase activity, forming ADP-ribosylated ATP (ADPr-ATP) and ADPr-ADPR (di-ADPR), respectively. Specific binding of ADPr-ATP or di-ADPR allosterically promotes EDS1-SAG101 interaction with helper NLR N requirement gene 1A (NRG1A) in vitro and in planta. Our data reveal an enzymatic activity of TIRs that enables specific activation of the EDS1-SAG101-NRG1 immunity branch.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Jia, AolinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Huang, ShijiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Song, WenUNSPECIFIEDorcid.org/0000-0002-9498-2409UNSPECIFIED
Wang, JunliUNSPECIFIEDorcid.org/0000-0001-8825-3894UNSPECIFIED
Meng, YonggangUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sun, YueUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Xu, LinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Laessle, HenrietteUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jirschitzka, JanUNSPECIFIEDorcid.org/0000-0003-1007-4700UNSPECIFIED
Hou, JiaoUNSPECIFIEDorcid.org/0000-0002-2945-1791UNSPECIFIED
Zhang, TiantianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yu, WenquanUNSPECIFIEDorcid.org/0000-0002-3711-0006UNSPECIFIED
Hessler, GiulianaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Li, ErtongUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ma, ShoucaiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yu, DongliUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gebauer, JanUNSPECIFIEDorcid.org/0000-0002-3989-4748UNSPECIFIED
Baumann, UlrichUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Liu, XiaohuiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Han, ZhifuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chang, JunbiaoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Parker, Jane E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chai, JijieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-682156
DOI: 10.1126/science.abq8180
Journal or Publication Title: Science
Volume: 377
Number: 6605
Page Range: S. 488 - 497
Date: 2022
Publisher: AMER ASSOC ADVANCEMENT SCIENCE
Place of Publication: WASHINGTON
ISSN: 1095-9203
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
TNL-MEDIATED IMMUNITY; CELL-DEATH; PATHOGEN EFFECTOR; DEFENSE; COMPLEX; DOMAIN; RECOGNITION; RECEPTORS; PROTEINS; EDS1Multiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/68215

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