Universität zu Köln

Karlsen, Anders ORCID: 0000-0002-9356-1346, Gonzalez-Franquesa, Alba ORCID: 0000-0001-9081-753X, Jakobsen, Jens R., Krogsgaard, Michael R., Koch, Manuel ORCID: 0000-0002-2962-7814, Kjaer, Michael, Schiaffino, Stefano, Mackey, Abigail L. and Deshmukh, Atul S. (2022). The proteomic profile of the human myotendinous junction. iScience, 25 (2). CAMBRIDGE: CELL PRESS. ISSN 2589-0042

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Abstract

Proteomics analysis of skeletal muscle has recently progressed from whole muscle tissue to single myofibers. Here, we further focus on a specific myofiber domain crucial for force transmission from muscle to tendon, the myotendinous junction (MTJ). To overcome the anatomical constraints preventing the isolation of pure MTJs, we performed in-depth analysis of the MTJ by progressive removal of the muscle component in semitendinosus muscle-tendon samples. Using detergents with increasing stringency, we quantified >3000 proteins across all samples, and identified 112 significantly enriched MTJ proteins, including 24 known MTJ-enriched proteins. Of the 88 novel MTJ markers, immunofluorescence analysis confirmed the presence of tetraspanin-24 (CD151), kindlin-2 (FERMT2), cartilage intermediate layer protein 1 (CILP), and integrin-alpha10 (ITGA10), at the human MTJ. Together, these human data constitute the first detailed MTJ proteomics resource that will contribute to advance understanding of the biology of the MTJ and its failure in conditions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Karlsen, Anders UNSPECIFIED orcid.org/0000-0002-9356-1346 UNSPECIFIED Gonzalez-Franquesa, Alba UNSPECIFIED orcid.org/0000-0001-9081-753X UNSPECIFIED Jakobsen, Jens R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Krogsgaard, Michael R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Manuel UNSPECIFIED orcid.org/0000-0002-2962-7814 UNSPECIFIED Kjaer, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Schiaffino, Stefano UNSPECIFIED UNSPECIFIED UNSPECIFIED Mackey, Abigail L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Deshmukh, Atul S. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-682637
DOI:	10.1016/j.isci.2022.103836
Journal or Publication Title:	iScience
Volume:	25
Number:	2
Date:	2022
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2589-0042
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN SKELETAL-MUSCLE; COMPUTATIONAL PLATFORM; BINDING INTEGRINS; PROTEIN; COMPONENT; CILP; ALPHA-7-BETA-1; LOCALIZATION; EXPRESSION; PATHWAYS Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68263