Universität zu Köln

Nemec, Alexandr ORCID: 0000-0003-0608-9598, Radolfova-Krizova, Lenka, Maixnerova, Martina, Shestivska, Violetta, Spanelova, Petra and Higgins, Paul G. (2022). Acinetobacter silvestris sp. nov. discovered in forest ecosystems in Czechia. Int. J. Syst. Evol. Microbiol., 72 (4). LONDON: MICROBIOLOGY SOC. ISSN 1466-5034

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Abstract

We investigated a taxonomically novel group of the genus Acinetobacter, which included five strains isolated from soil and water samples collected in preserved forest areas in Czechia between 2013 and 2021. The whole-genome sequences of the strains were 3.1-3.2 Mb in size, with G+C contents of 38.0-38.2mol%. Core genome-based phylogenetic analysis showed that they formed a compact and deeply branched Glade within the genus. The genomic average nucleotide identity based on BLAST/digital DNA-DNA hybridization values for the novel strains were 99.2-99.6%/95.2-98.4%, whereas those between the novel strains and the type strains of the known Acinetobacter species reached <78%/<24%. The results of the genus-wide analysis of whole-cell MALDI-TOF mass spectra supported the sharp distinctness of the group. The five strains were non-glucose acidifying. nonhaemolytic, nonproteolytic and growing at 28 degrees C. but not at 32 degrees C; they assimilated acetate, benzoate, ethanol, L-histidine, 4-hydroxybenzoate, DL-lactate and malonate but not 4-aminobutyrate, L-aspartate or 2,3-butanediol; this phenotype is unique among the known Acinetobacter species. We conclude that the five strains represent a novel environmental species, for which the name Acinetobacter silvestris sp. nov. is proposed, with the type strain ANC 4999(T) (=CCM 920(T)=CCUG 7587(T)=CNCTC 8124(T)).

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Nemec, Alexandr UNSPECIFIED orcid.org/0000-0003-0608-9598 UNSPECIFIED Radolfova-Krizova, Lenka UNSPECIFIED UNSPECIFIED UNSPECIFIED Maixnerova, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Shestivska, Violetta UNSPECIFIED UNSPECIFIED UNSPECIFIED Spanelova, Petra UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-682991
DOI:	10.1099/ijsem.0.005383
Journal or Publication Title:	Int. J. Syst. Evol. Microbiol.
Volume:	72
Number:	4
Date:	2022
Publisher:	MICROBIOLOGY SOC
Place of Publication:	LONDON
ISSN:	1466-5034
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NATURAL SOIL; DIVERSIFICATION; WIDESPREAD Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68299