Universität zu Köln

Zacharaki, Vasiliki ORCID: 0000-0002-5543-2332, Ponnu, Jathish ORCID: 0000-0002-3276-7068, Crepin, Nathalie ORCID: 0000-0002-5772-4216, Langenecker, Tobias, Hagmann, Joerg, Skorzinski, Noemi ORCID: 0000-0002-1334-385X, Musialak-Lange, Magdalena ORCID: 0000-0002-0388-8960, Wahl, Vanessa ORCID: 0000-0001-7421-8801, Rolland, Filip ORCID: 0000-0003-2601-0752 and Schmid, Markus ORCID: 0000-0002-0068-2967 (2022). Impaired KIN10 function restores developmental defects in the Arabidopsis trehalose 6-phosphate synthase1 (tps1) mutant. New Phytol., 235 (1). S. 220 - 234. HOBOKEN: WILEY. ISSN 1469-8137

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Abstract

Sensing carbohydrate availability is essential for plants to coordinate their growth and development. In Arabidopsis thaliana, TREHALOSE 6-PHOSPHATE SYNTHASE 1 (TPS1) and its product, trehalose 6-phosphate (T6P), are important for the metabolic control of development. tps1 mutants are embryo-lethal and unable to flower when embryogenesis is rescued. T6P regulates development in part through inhibition of SUCROSE NON-FERMENTING1 RELATED KINASE1 (SnRK1). Here, we explored the role of SnRK1 in T6P-mediated plant growth and development using a combination of a mutant suppressor screen and genetic, cellular and transcriptomic approaches. We report nonsynonymous amino acid substitutions in the catalytic KIN10 and regulatory SNF4 subunits of SnRK1 that can restore both embryogenesis and flowering of tps1 mutant plants. The identified SNF4 point mutations disrupt the interaction with the catalytic subunit KIN10. Contrary to the common view that the two A. thaliana SnRK1 catalytic subunits act redundantly, we found that loss-of-function mutations in KIN11 are unable to restore embryogenesis and flowering, highlighting the important role of KIN10 in T6P signalling.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zacharaki, Vasiliki UNSPECIFIED orcid.org/0000-0002-5543-2332 UNSPECIFIED Ponnu, Jathish UNSPECIFIED orcid.org/0000-0002-3276-7068 UNSPECIFIED Crepin, Nathalie UNSPECIFIED orcid.org/0000-0002-5772-4216 UNSPECIFIED Langenecker, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Hagmann, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED Skorzinski, Noemi UNSPECIFIED orcid.org/0000-0002-1334-385X UNSPECIFIED Musialak-Lange, Magdalena UNSPECIFIED orcid.org/0000-0002-0388-8960 UNSPECIFIED Wahl, Vanessa UNSPECIFIED orcid.org/0000-0001-7421-8801 UNSPECIFIED Rolland, Filip UNSPECIFIED orcid.org/0000-0003-2601-0752 UNSPECIFIED Schmid, Markus UNSPECIFIED orcid.org/0000-0002-0068-2967 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-683041
DOI:	10.1111/nph.18104
Journal or Publication Title:	New Phytol.
Volume:	235
Number:	1
Page Range:	S. 220 - 234
Date:	2022
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1469-8137
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROTEIN-KINASE; TREHALOSE 6-PHOSPHATE; ARABIDOPSIS-THALIANA; ENERGY SENSOR; TREHALOSE-6-PHOSPHATE SYNTHASE-1; VEGETATIVE GROWTH; SNRK1; EXPRESSION; STRESS; IDENTIFICATION Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68304