Universität zu Köln

Valencia, Karmele ORCID: 0000-0002-2882-7427, Sainz, Cristina, Bertolo, Cristina, de Biurrun, Gabriel ORCID: 0000-0003-2305-0724, Agorreta, Jackeline, Azpilikueta, Arantza, Larrayoz, Marta, Bosco, Graziella ORCID: 0000-0001-6601-4518, Zandueta, Carolina, Redrado, Miriam ORCID: 0000-0003-0120-0779, Redin, Esther, Exposito, Francisco, Serrano, Diego ORCID: 0000-0002-3729-9195, Echepare, Mirari, Ajona, Daniel ORCID: 0000-0001-5517-9165, Melero, Ignacio ORCID: 0000-0002-1360-348X, Pio, Ruben ORCID: 0000-0002-6831-6111, Thomas, Roman, Calvo, Alfonso ORCID: 0000-0003-4074-4242 and Montuenga, Luis M. (2022). Two cell line models to study multiorganic metastasis and immunotherapy in lung squamous cell carcinoma. Dis. Model. Mech., 15 (1). CAMBRIDGE: COMPANY BIOLOGISTS LTD. ISSN 1754-8411

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Abstract

There is a paucity of adequate mouse models and cell lines available to study lung squamous cell carcinoma (LUSC). We have generated and characterized two models of phenotypically different transplantable LUSC cell lines, i.e. UN-SCC679 and UN-SCC680, derived from A/J mice that had been chemically induced with N-nitroso-tris-chloroethylurea (NTCU). Furthermore, we genetically characterized and compared both LUSC cell lines by performing whole-exome and RNA sequencing. These experiments revealed similar genetic and transcriptomic patterns that may correspond to the classic LUSC human subtype. In addition, we compared the immune landscape generated by both tumor cells lines in vivo and assessed their response to immune checkpoint inhibition. The differences between the two cell lines are a good model for the remarkable heterogeneity of human squamous cell carcinoma. Study of the metastatic potential of these models revealed that both cell lines represent the organotropism of LUSC in humans, i.e. affinity to the brain, bones, liver and adrenal glands. In summary, we have generated valuable cell line tools for LUSC research, which recapitulates the complexity of the human disease.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Valencia, Karmele UNSPECIFIED orcid.org/0000-0002-2882-7427 UNSPECIFIED Sainz, Cristina UNSPECIFIED UNSPECIFIED UNSPECIFIED Bertolo, Cristina UNSPECIFIED UNSPECIFIED UNSPECIFIED de Biurrun, Gabriel UNSPECIFIED orcid.org/0000-0003-2305-0724 UNSPECIFIED Agorreta, Jackeline UNSPECIFIED UNSPECIFIED UNSPECIFIED Azpilikueta, Arantza UNSPECIFIED UNSPECIFIED UNSPECIFIED Larrayoz, Marta UNSPECIFIED UNSPECIFIED UNSPECIFIED Bosco, Graziella UNSPECIFIED orcid.org/0000-0001-6601-4518 UNSPECIFIED Zandueta, Carolina UNSPECIFIED UNSPECIFIED UNSPECIFIED Redrado, Miriam UNSPECIFIED orcid.org/0000-0003-0120-0779 UNSPECIFIED Redin, Esther UNSPECIFIED UNSPECIFIED UNSPECIFIED Exposito, Francisco UNSPECIFIED UNSPECIFIED UNSPECIFIED Serrano, Diego UNSPECIFIED orcid.org/0000-0002-3729-9195 UNSPECIFIED Echepare, Mirari UNSPECIFIED UNSPECIFIED UNSPECIFIED Ajona, Daniel UNSPECIFIED orcid.org/0000-0001-5517-9165 UNSPECIFIED Melero, Ignacio UNSPECIFIED orcid.org/0000-0002-1360-348X UNSPECIFIED Pio, Ruben UNSPECIFIED orcid.org/0000-0002-6831-6111 UNSPECIFIED Thomas, Roman UNSPECIFIED UNSPECIFIED UNSPECIFIED Calvo, Alfonso UNSPECIFIED orcid.org/0000-0003-4074-4242 UNSPECIFIED Montuenga, Luis M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-686016
DOI:	10.1242/dmm.049137
Journal or Publication Title:	Dis. Model. Mech.
Volume:	15
Number:	1
Date:	2022
Publisher:	COMPANY BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	1754-8411
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TUMOR-SUPPRESSOR; PD-1 BLOCKADE; CANCER; MUTATIONS; GENE; MICE Multiple languages Cell Biology; Pathology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68601