Universität zu Köln

Boyen, Jens ORCID: 0000-0001-5005-7724, Ribes-Navarro, Alberto ORCID: 0000-0002-8293-7168, Kabeya, Naoki, Monroig, Oscar ORCID: 0000-0001-8712-0440, Rigaux, Annelien, Fink, Patrick ORCID: 0000-0002-5927-8977, Habluetzel, Pascal I., Navarro, Juan Carlos and De Troch, Marleen ORCID: 0000-0002-6800-0299 (2023). Functional characterization reveals a diverse array of metazoan fatty acid biosynthesis genes. Mol. Ecol., 32 (4). S. 970 - 983. HOBOKEN: WILEY. ISSN 1365-294X

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Abstract

Long-chain (>= C-20) polyunsaturated fatty acids (LC-PUFAs) are physiologically important fatty acids for most animals, including humans. Although most LC-PUFA production occurs in aquatic primary producers such as microalgae, recent research indicates the ability of certain groups of (mainly marine) invertebrates for endogenous LC-PUFA biosynthesis and/or bioconversion from dietary precursors. The genetic pathways for and mechanisms behind LC-PUFA biosynthesis remain unknown in many invertebrates to date, especially in non-model species. However, the numerous genomic and transcriptomic resources currently available can contribute to our knowledge of the LC-PUFA biosynthetic capabilities of metazoans. Within our previously generated transcriptome of the benthic harpacticoid copepod Platychelipus littoralis, we detected expression of one methyl-end desaturase, one front-end desaturase, and seven elongases, key enzymes responsible for LC-PUFA biosynthesis. To demonstrate their functionality, we characterized eight of them using heterologous expression in yeast. The P. littoralis methyl-end desaturase has Delta 15/17/19 desaturation activity, enabling biosynthesis of alpha-linolenic acid, eicosapentaenoic acid and docosahexaenoic acid (DHA) from 18:2 n-6, 20:4 n-6 and 22:5 n-6, respectively. Its front-end desaturase has Delta 4 desaturation activity from 22:5 n-3 to DHA, implying that P. littoralis has multiple pathways to produce this physiologically important fatty acid. All studied P. littoralis elongases possess varying degrees of elongation activity for saturated and unsaturated fatty acids, producing aliphatic hydrocarbon chains with lengths of up to 30 carbons. Our investigation revealed a functionally diverse range of fatty acid biosynthesis genes in copepods, which highlights the need to scrutinize the role that primary consumers could perform in providing essential nutrients to upper trophic levels.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Boyen, Jens UNSPECIFIED orcid.org/0000-0001-5005-7724 UNSPECIFIED Ribes-Navarro, Alberto UNSPECIFIED orcid.org/0000-0002-8293-7168 UNSPECIFIED Kabeya, Naoki UNSPECIFIED UNSPECIFIED UNSPECIFIED Monroig, Oscar UNSPECIFIED orcid.org/0000-0001-8712-0440 UNSPECIFIED Rigaux, Annelien UNSPECIFIED UNSPECIFIED UNSPECIFIED Fink, Patrick UNSPECIFIED orcid.org/0000-0002-5927-8977 UNSPECIFIED Habluetzel, Pascal I. UNSPECIFIED UNSPECIFIED UNSPECIFIED Navarro, Juan Carlos UNSPECIFIED UNSPECIFIED UNSPECIFIED De Troch, Marleen UNSPECIFIED orcid.org/0000-0002-6800-0299 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-686489
DOI:	10.1111/mec.16808
Journal or Publication Title:	Mol. Ecol.
Volume:	32
Number:	4
Page Range:	S. 970 - 983
Date:	2023
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-294X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DESATURASE-LIKE GENE; HARPACTICOID COPEPODS; LIVE FOOD; TISBE SP; TEMPERATURE; EXPRESSION; ELONGASES; BIOCONVERSION; ADAPTATION; INSIGHTS Multiple languages Biochemistry & Molecular Biology; Ecology; Evolutionary Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68648