Universität zu Köln

Pollegioni, Paola ORCID: 0000-0001-6388-1931, Persampieri, Tania, Minuz, Roxana L., Bucci, Alessandro, Trusso, Alessandro, Di Martino, Salvatore, Leo, Chiara, Bruttini, Marco ORCID: 0000-0003-1063-2522, Ciolfi, Marco, Waldvogel, Ann-Marie, Tripet, Frederic, Simoni, Alekos ORCID: 0000-0001-8629-721X, Crisanti, Andrea and Mueller, Ruth (2023). Introgression of a synthetic sex ratio distortion transgene into different genetic backgrounds of Anopheles coluzzii. Insect Mol. Biol., 32 (1). S. 56 - 69. HOBOKEN: WILEY. ISSN 1365-2583

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Abstract

The development of genetically modified mosquitoes (GMM) and their subsequent field release offers innovative approaches for vector control of malaria. A non-gene drive self-limiting male-bias Ag(PMB)1 strain has been developed in a 47-year-old laboratory G3 strain of Anopheles gambiae s.l. When Ag(PMB)1 males are crossed to wild-type females, expression of the endonuclease I-PpoI during spermatogenesis causes the meiotic cleavage of the X chromosome in sperm cells, leading to fertile offspring with a 95% male bias. However, World Health Organization states that the functionality of the transgene could differ when inserted in different genetic backgrounds of Anopheles coluzzii which is currently a predominant species in several West-African countries and thus a likely recipient for a potential release of self-limiting GMMs. In this study, we introgressed the transgene from the donor Ag(PMB)1 by six serial backcrosses into two recipient colonies of An. coluzzii that had been isolated in Mali and Burkina Faso. Scans of informative Single Nucleotide Polymorphism (SNP) markers and whole-genome sequencing analysis revealed a nearly complete introgression of chromosomes 3 and X, but a remarkable genomic divergence in a large region of chromosome 2 between the later backcrossed (BC6) transgenic offspring and the recipient paternal strains. These findings suggested to extend the backcrossing breeding strategy beyond BC6 generation and increasing the introgression efficiency of critical regions that have ecological and epidemiological implications through the targeted selection of specific markers. Disregarding differential introgression efficiency, we concluded that the phenotype of the sex ratio distorter is stable in the BC6 introgressed An. coluzzii strains.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Pollegioni, Paola UNSPECIFIED orcid.org/0000-0001-6388-1931 UNSPECIFIED Persampieri, Tania UNSPECIFIED UNSPECIFIED UNSPECIFIED Minuz, Roxana L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bucci, Alessandro UNSPECIFIED UNSPECIFIED UNSPECIFIED Trusso, Alessandro UNSPECIFIED UNSPECIFIED UNSPECIFIED Di Martino, Salvatore UNSPECIFIED UNSPECIFIED UNSPECIFIED Leo, Chiara UNSPECIFIED UNSPECIFIED UNSPECIFIED Bruttini, Marco UNSPECIFIED orcid.org/0000-0003-1063-2522 UNSPECIFIED Ciolfi, Marco UNSPECIFIED UNSPECIFIED UNSPECIFIED Waldvogel, Ann-Marie UNSPECIFIED UNSPECIFIED UNSPECIFIED Tripet, Frederic UNSPECIFIED UNSPECIFIED UNSPECIFIED Simoni, Alekos UNSPECIFIED orcid.org/0000-0001-8629-721X UNSPECIFIED Crisanti, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Ruth UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-687167
DOI:	10.1111/imb.12813
Journal or Publication Title:	Insect Mol. Biol.
Volume:	32
Number:	1
Page Range:	S. 56 - 69
Date:	2023
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-2583
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language AFRICAN MALARIA VECTOR; MOLECULAR-FORMS; REPRODUCTIVE ISOLATION; GAMBIAE DIPTERA; 2LA INVERSION; MOSQUITO; COMPLEX; RESISTANCE; MALES; FLOW Multiple languages Biochemistry & Molecular Biology; Entomology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68716