Universität zu Köln

Kanber, Deniz, Woestefeld, Julia, Doepper, Hannah, Bozet, Morgane, Brenzel, Alexandra, Altmueller, Janine, Kilpert, Fabian, Lohmann, Dietmar, Pommerenke, Claudia and Steenpass, Laura (2022). RB1-Negative Retinal Organoids Display Proliferation of Cone Photoreceptors and Loss of Retinal Differentiation. Cancers, 14 (9). BASEL: MDPI. ISSN 2072-6694

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Abstract

Retinoblastoma is a tumor of the eye's retina, which is the very specialized tissue responsible for vision. In 98% of cases, the tumor is caused by inactivation of the RB1 gene. Due to lack of material and models, the understanding of RB1 mutations in tumor development is still unsatisfactory. We aimed to establish a human laboratory model for retinoblastoma. While differentiating stem cells with a mutation in RB1 into retina, we observed reduced differentiation potential but enhanced proliferation-general hallmarks of tumor development. The gene expression signature in the model resembled that of tumor material. This approach now enables research on retinoblastoma and probably therapy in the correct tissue, the human retina. Retinoblastoma is a tumor of the eye in children under the age of five caused by biallelic inactivation of the RB1 tumor suppressor gene in maturing retinal cells. Cancer models are essential for understanding tumor development and in preclinical research. Because of the complex organization of the human retina, such models were challenging to develop for retinoblastoma. Here, we present an organoid model based on differentiation of human embryonic stem cells into neural retina after inactivation of RB1 by CRISPR/Cas9 mutagenesis. Wildtype and RB1 heterozygous mutant retinal organoids were indistinguishable with respect to morphology, temporal development of retinal cell types and global mRNA expression. However, loss of pRB resulted in spatially disorganized organoids and aberrant differentiation, indicated by disintegration of organoids beyond day 130 of differentiation and depletion of most retinal cell types. Only cone photoreceptors were abundant and continued to proliferate, supporting these as candidate cells-of-origin for retinoblastoma. Transcriptome analysis of RB1 knockout organoids and primary retinoblastoma revealed gain of a retinoblastoma expression signature in the organoids, characterized by upregulation of RBL1 (p107), MDM2, DEK, SYK and HELLS. In addition, genes related to immune response and extracellular matrix were specifically upregulated in RBI-negative organoids. In vitro retinal organoids therefore display some features associated with retinoblastoma and, so far, represent the only valid human cancer model for the development of this disease.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kanber, Deniz UNSPECIFIED UNSPECIFIED UNSPECIFIED Woestefeld, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Doepper, Hannah UNSPECIFIED UNSPECIFIED UNSPECIFIED Bozet, Morgane UNSPECIFIED UNSPECIFIED UNSPECIFIED Brenzel, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Altmueller, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Kilpert, Fabian UNSPECIFIED UNSPECIFIED UNSPECIFIED Lohmann, Dietmar UNSPECIFIED UNSPECIFIED UNSPECIFIED Pommerenke, Claudia UNSPECIFIED UNSPECIFIED UNSPECIFIED Steenpass, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-687305
DOI:	10.3390/cancers14092166
Journal or Publication Title:	Cancers
Volume:	14
Number:	9
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2072-6694
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GENOMIC ALTERATIONS; GENE-EXPRESSION; R PACKAGE; RETINOBLASTOMA; RB1; GENERATION; TUMOR; MUTATIONS; DELETION; ORIGIN Multiple languages Oncology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68730