Goebel, Heike ORCID: 0000-0002-0171-7025, Koeditz, Barbara, Huerta, Manuel, Kameri, Ersen, Nestler, Tim, Kamphausen, Thomas ORCID: 0000-0002-2799-4418, Friemann, Johannes, Hamdorf, Matthias ORCID: 0000-0003-3744-4548, Ohrmann, Timo, Koehler, Philipp, Cornely, Oliver A. ORCID: 0000-0001-9599-3137, Montesinos-Rongen, Manuel, Nicol, David, Schorle, Hubert ORCID: 0000-0001-8272-0076, Boor, Peter ORCID: 0000-0001-9921-4284, Quaas, Alexander, Pallasch, Christian, Heidenreich, Axel and von Brandenstein, Melanie (2022). COVID-19 Infection Induce miR-371a-3p Upregulation Resulting in Influence on Male Fertility. Biomedicines, 10 (4). BASEL: MDPI. ISSN 2227-9059

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Abstract

In December 2019, the first case of COVID-19 was reported and since then several groups have already published that the virus can be present in the testis. To study the influence of SARSCoV-2 which cause a dysregulation of the androgen receptor (AR) level, thereby leading to fertility problems and inducing germ cell testicular changes in patients after the infection. Formalin-Fixed-Paraffin-Embedded (FFPE) testicular samples from patients who died with or as a result of COVID-19 (n = 32) with controls (n = 6), inflammatory changes (n = 9), seminoma with/without metastasis (n = 11) compared with healthy biopsy samples (n = 3) were analyzed and compared via qRT-PCR for the expression of miR-371a-3p. An immunohistochemical analysis (IHC) and ELISA were performed in order to highlight the miR-371a-3p targeting the AR. Serum samples of patients with mild or severe COVID-19 symptoms (n = 34) were analyzed for miR-371a-3p expression. In 70% of the analyzed postmortem testicular tissue samples, a significant upregulation of the miR-371a-3p was detected, and 75% of the samples showed a reduced spermatogenesis. In serum samples, the upregulation of the miR-371a-3p was also detectable. The upregulation of the miR-371a-3p is responsible for the downregulation of the AR in SARS-CoV-2-positive patients, resulting in decreased spermatogenesis. Since the dysregulation of the AR is associated with infertility, further studies have to confirm if the identified dysregulation is regressive after a declining infection.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Goebel, HeikeUNSPECIFIEDorcid.org/0000-0002-0171-7025UNSPECIFIED
Koeditz, BarbaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Huerta, ManuelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kameri, ErsenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nestler, TimUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kamphausen, ThomasUNSPECIFIEDorcid.org/0000-0002-2799-4418UNSPECIFIED
Friemann, JohannesUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hamdorf, MatthiasUNSPECIFIEDorcid.org/0000-0003-3744-4548UNSPECIFIED
Ohrmann, TimoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koehler, PhilippUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cornely, Oliver A.UNSPECIFIEDorcid.org/0000-0001-9599-3137UNSPECIFIED
Montesinos-Rongen, ManuelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nicol, DavidUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schorle, HubertUNSPECIFIEDorcid.org/0000-0001-8272-0076UNSPECIFIED
Boor, PeterUNSPECIFIEDorcid.org/0000-0001-9921-4284UNSPECIFIED
Quaas, AlexanderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pallasch, ChristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Heidenreich, AxelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
von Brandenstein, MelanieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-687760
DOI: 10.3390/biomedicines10040858
Journal or Publication Title: Biomedicines
Volume: 10
Number: 4
Date: 2022
Publisher: MDPI
Place of Publication: BASEL
ISSN: 2227-9059
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
GERM-CELL TUMORS; ANDROGEN LEVELS; MICRORNASMultiple languages
Biochemistry & Molecular Biology; Medicine, Research & Experimental; Pharmacology & PharmacyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/68776

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