Universität zu Köln

Dalimot, Jill Jasmine, Smith, Ilka Mc Cormick, Gerkrath, Jasmin, Hartmann, Sylvia, Cornely, Oliver A. ORCID: 0000-0001-9599-3137, Lee, Soo Chan ORCID: 0000-0002-7792-5947, Heitman, Joseph and Rickerts, Volker ORCID: 0000-0002-6154-4920 (2022). Identification of Mucormycosis by Fluorescence In Situ Hybridization Targeting Ribosomal RNA in Tissue Samples. J. Fungi, 8 (3). BASEL: MDPI. ISSN 2309-608X

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Abstract

Mucormycosis is an invasive fungal infection associated with high mortality, partly due to delayed diagnosis and inadequate empiric therapy. As fungal cultures often fail to grow Mucorales, identification of respective hyphae in tissue is frequently needed for diagnosis but may be challenging. We studied fluorescence in situ hybridization (FISH) targeting specific regions of the fungal ribosomal RNA (rRNA) of Mucorales to improve diagnosis of mucormycosis from tissue samples. We generated a probe combination specifically targeting Mucorales. Probe specificity was verified in silico and using cultivated fungi. Mucorales hyphae in tissue of a mouse model demonstrated a bright cytoplasmatic hybridization signal. In tissue samples of patients with mucormycosis, a positive signal was seen in 7 of 12 (58.3%) samples. However, autofluorescence in 3 of 7 (42.9%) samples impaired the diagnostic yield. Subsequent experiments suggested that availability of nutrients and antifungal therapy may impact on the FISH signal obtained with Mucorales hyphae. Diagnosis of mucormycosis from tissue might be improved by rRNA FISH in a limited number of cases only. FISH signals may reflect different physiological states of fungi in tissue. Further studies are needed to define the value of FISH to diagnose mucormycosis from other clinical samples.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Dalimot, Jill Jasmine UNSPECIFIED UNSPECIFIED UNSPECIFIED Smith, Ilka Mc Cormick UNSPECIFIED UNSPECIFIED UNSPECIFIED Gerkrath, Jasmin UNSPECIFIED UNSPECIFIED UNSPECIFIED Hartmann, Sylvia UNSPECIFIED UNSPECIFIED UNSPECIFIED Cornely, Oliver A. UNSPECIFIED orcid.org/0000-0001-9599-3137 UNSPECIFIED Lee, Soo Chan UNSPECIFIED orcid.org/0000-0002-7792-5947 UNSPECIFIED Heitman, Joseph UNSPECIFIED UNSPECIFIED UNSPECIFIED Rickerts, Volker UNSPECIFIED orcid.org/0000-0002-6154-4920 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-688419
DOI:	10.3390/jof8030289
Journal or Publication Title:	J. Fungi
Volume:	8
Number:	3
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2309-608X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EPIDEMIOLOGY Multiple languages Microbiology; Mycology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68841