Universität zu Köln

Rawat, Anamika A., Hartmann, Michael ORCID: 0000-0003-4828-958X, Harzen, Anne ORCID: 0000-0001-7370-4939, Lugan, Raphael, Stolze, Sara Christina ORCID: 0000-0002-1421-9703, Forzani, Celine, Abts, Laura, Reissenweber, Sophie, Rayapuram, Naganand, Nakagami, Hirofumi, Zeier, Jurgen and Hirt, Heribert (2023). OXIDATIVE SIGNAL-INDUCIBLE1 induces immunity by coordinating N-hydroxypipecolic acid, salicylic acid, and camalexin synthesis. New Phytol., 237 (4). S. 1285 - 1302. HOBOKEN: WILEY. ISSN 1469-8137

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Abstract

Expression of OXIDATIVE SIGNAL-INDUCIBLE1 (OXI1) is induced by a number of stress conditions and regulates the interaction of plants with pathogenic and beneficial microbes. In this work, we generated Arabidopsis OXI1 knockout and genomic OXI1 overexpression lines and show by transcriptome, proteome, and metabolome analysis that OXI1 triggers ALD1, SARD4, and FMO1 expressions to promote the biosynthesis of pipecolic acid (Pip) and N-hydroxypipecolic acid (NHP). OXI1 contributes to enhanced immunity by induced SA biosynthesis via CBP60g-induced expression of SID2 and camalexin accumulation via WRKY33-targeted transcription of PAD3. OXI1 regulates genes involved in reactive oxygen species (ROS) generation such as RbohD and RbohF. OXI1 knock out plants show enhanced expression of nuclear and chloroplast genes of photosynthesis and enhanced growth under ambient conditions, while OXI1 overexpressing plants accumulate NHP, SA, camalexin, and ROS and show a gain-of-function (GOF) cell death phenotype and enhanced pathogen resistance. The OXI1 GOF phenotypes are completely suppressed when compromising N-hydroxypipecolic acid (NHP) synthesis in the fmo1 or ald1 background, showing that OXI1 regulation of immunity is mediated via the NHP pathway. Overall, these results show that OXI1 plays a key role in basal and effector-triggered plant immunity by regulating defense and programmed cell death via biosynthesis of salicylic acid, N-hydroxypipecolic acid, and camalexin.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rawat, Anamika A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hartmann, Michael UNSPECIFIED orcid.org/0000-0003-4828-958X UNSPECIFIED Harzen, Anne UNSPECIFIED orcid.org/0000-0001-7370-4939 UNSPECIFIED Lugan, Raphael UNSPECIFIED UNSPECIFIED UNSPECIFIED Stolze, Sara Christina UNSPECIFIED orcid.org/0000-0002-1421-9703 UNSPECIFIED Forzani, Celine UNSPECIFIED UNSPECIFIED UNSPECIFIED Abts, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED Reissenweber, Sophie UNSPECIFIED UNSPECIFIED UNSPECIFIED Rayapuram, Naganand UNSPECIFIED UNSPECIFIED UNSPECIFIED Nakagami, Hirofumi UNSPECIFIED UNSPECIFIED UNSPECIFIED Zeier, Jurgen UNSPECIFIED UNSPECIFIED UNSPECIFIED Hirt, Heribert UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-689344
DOI:	10.1111/nph.18592
Journal or Publication Title:	New Phytol.
Volume:	237
Number:	4
Page Range:	S. 1285 - 1302
Date:	2023
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1469-8137
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SYSTEMIC ACQUIRED-RESISTANCE; PIPECOLIC ACID; PLANT IMMUNITY; PSEUDOMONAS-SYRINGAE; DEFENSE RESPONSES; PATHOGEN DEFENSE; PROTEIN-KINASE; BIOSYNTHESIS; STRESS; OXI1 Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68934