Universität zu Köln

Hwang, Youra ORCID: 0000-0002-6368-6113, Han, Soeun ORCID: 0000-0001-5570-2307, Yoo, Chan Yul, Hong, Liu, You, Chenjiang, Le, Brandon H., Shi, Hui, Zhong, Shangwei, Hoecker, Ute, Chen, Xuemei ORCID: 0000-0002-5209-1157 and Chen, Meng ORCID: 0000-0003-0351-5897 (2022). Anterograde signaling controls plastid transcription via sigma factors separately from nuclear photosynthesis genes. Nat. Commun., 13 (1). BERLIN: NATURE PORTFOLIO. ISSN 2041-1723

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Abstract

Light initiates chloroplast biogenesis in Arabidopsis by eliminating PHYTOCHROME-INTERACTING transcription FACTORs (PIFs), which in turn de-represses nuclear photosynthesis genes, and synchronously, generates a nucleus-to-plastid (anterograde) signal that activates the plastid-encoded bacterial-type RNA polymerase (PEP) to transcribe plastid photosynthesis genes. However, the identity of the anterograde signal remains frustratingly elusive. The main challenge has been the difficulty to distinguish regulators from the plethora of necessary components for plastid transcription and other essential chloroplast functions, such as photosynthesis. Here, we show that the genome-wide induction of nuclear photosynthesis genes is insufficient to activate the PEP. PEP inhibition is imposed redundantly by multiple PIFs and requires PIF3's activator activity. Among the nuclear-encoded components of the PEP holoenzyme, we identify four light-inducible, PIF-repressed sigma factors as anterograde signals. Together, our results elucidate that light-dependent inhibition of PIFs activates plastid photosynthesis genes via sigma factors as anterograde signals in parallel with the induction of nuclear photosynthesis genes. Photoreceptors in the nucleus control plastid transcription by utilizing sigma factors as nucleus-to-plastid signals in parallel with the light regulation of nuclear photosynthesis genes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hwang, Youra UNSPECIFIED orcid.org/0000-0002-6368-6113 UNSPECIFIED Han, Soeun UNSPECIFIED orcid.org/0000-0001-5570-2307 UNSPECIFIED Yoo, Chan Yul UNSPECIFIED UNSPECIFIED UNSPECIFIED Hong, Liu UNSPECIFIED UNSPECIFIED UNSPECIFIED You, Chenjiang UNSPECIFIED UNSPECIFIED UNSPECIFIED Le, Brandon H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Shi, Hui UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhong, Shangwei UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoecker, Ute UNSPECIFIED UNSPECIFIED UNSPECIFIED Chen, Xuemei UNSPECIFIED orcid.org/0000-0002-5209-1157 UNSPECIFIED Chen, Meng UNSPECIFIED orcid.org/0000-0003-0351-5897 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-689419
DOI:	10.1038/s41467-022-35080-0
Journal or Publication Title:	Nat. Commun.
Volume:	13
Number:	1
Date:	2022
Publisher:	NATURE PORTFOLIO
Place of Publication:	BERLIN
ISSN:	2041-1723
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LIGHT-DEPENDENT INTERACTION; CRYPTOCHROME 1 INTERACTS; PHYTOCHROME-A; CHLOROPLAST TRANSCRIPTION; RNA-POLYMERASE; BLUE-LIGHT; PIF3 PHOSPHORYLATION; ARABIDOPSIS; PHOTOMORPHOGENESIS; EXPRESSION Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68941