Zhao, Jierui ORCID: 0000-0002-6690-4040, Ma, Juncai, Kuenzl, Fabian, Picchianti, Lorenzo, De la Concepcion, Juan Carlos, Chen, Yixuan, Petsangouraki, Sofia ORCID: 0000-0001-7729-1334, Mohseni, Azadeh, Garcia-Leon, Marta, Gomez, Marta Salas, Giannini, Caterina, Gwennogan, Dubois, Kobylinska, Roksolana, Clavel, Marion, Schellmann, Swen, Jaillais, Yvon, Friml, Jiri, Kang, Byung-Ho and Dagdas, Yasin (2022). Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole. J. Cell Biol., 221 (12). NEW YORK: ROCKEFELLER UNIV PRESS. ISSN 1540-8140

Full text not available from this repository.

Abstract

Zhao et al. reveal amphisome compartments in plant cells. By characterizing the autophagy adaptor CFS1 that can interact with both ATG8, and the ESCRT-I complex subunit VPS23A; they show that autophagosomes are sorted at amphisomes before arriving their final destination, the central vacuole. Autophagosomes are double-membraned vesicles that traffic harmful or unwanted cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis has been extensively studied, autophagosome maturation, i.e., delivery and fusion with the vacuole, remains largely unknown in plants. Here, we have identified an autophagy adaptor, CFS1, that directly interacts with the autophagosome marker ATG8 and localizes on both membranes of the autophagosome. Autophagosomes form normally in Arabidopsis thaliana cfs1 mutants, but their delivery to the vacuole is disrupted. CFS1's function is evolutionarily conserved in plants, as it also localizes to the autophagosomes and plays a role in autophagic flux in the liverwort Marchantia polymorpha. CFS1 regulates autophagic flux by bridging autophagosomes with the multivesicular body-localized ESCRT-I component VPS23A, leading to the formation of amphisomes. Similar to CFS1-ATG8 interaction, disrupting the CFS1-VPS23A interaction blocks autophagic flux and renders plants sensitive to nitrogen starvation. Altogether, our results reveal a conserved vacuolar sorting hub that regulates autophagic flux in plants.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Zhao, JieruiUNSPECIFIEDorcid.org/0000-0002-6690-4040UNSPECIFIED
Ma, JuncaiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kuenzl, FabianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Picchianti, LorenzoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
De la Concepcion, Juan CarlosUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chen, YixuanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Petsangouraki, SofiaUNSPECIFIEDorcid.org/0000-0001-7729-1334UNSPECIFIED
Mohseni, AzadehUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Garcia-Leon, MartaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gomez, Marta SalasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Giannini, CaterinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gwennogan, DuboisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kobylinska, RoksolanaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Clavel, MarionUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schellmann, SwenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jaillais, YvonUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Friml, JiriUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kang, Byung-HoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dagdas, YasinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-691760
DOI: 10.1083/jcb.202203139
Journal or Publication Title: J. Cell Biol.
Volume: 221
Number: 12
Date: 2022
Publisher: ROCKEFELLER UNIV PRESS
Place of Publication: NEW YORK
ISSN: 1540-8140
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
AGROBACTERIUM-MEDIATED TRANSFORMATION; INDUCED MITOPHAGY; ATPASE ACTIVITY; ARABIDOPSIS; PROTEIN; TRAFFICKING; DEGRADATION; BIOGENESIS; METABOLISM; NUCLEOTIDEMultiple languages
Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/69176

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item