Universität zu Köln

Tyc, Olaf ORCID: 0000-0001-9949-0975, Kulkarni, Purva, Ossowicki, Adam ORCID: 0000-0003-1903-9688, Tracanna, Vittorio, Medema, Marnix H. ORCID: 0000-0002-2191-2821, van Baarlen, Peter ORCID: 0000-0003-3530-5472, van IJcken, W. F. J., Verhoeven, Koen J. F. and Garbeva, Paolina (2023). Exploring the Interspecific Interactions and the Metabolome of the Soil Isolate Hylemonella gracilis. mSystems, 8 (1). WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 2379-5077

Full text not available from this repository.

Abstract

Microbial community analysis of aquatic environments showed that an important component of its microbial diversity consists of bacteria with cell sizes of similar to 0.1 mu m. Such small bacteria can show genomic reductions and metabolic dependencies with other bacteria. However, so far, no study has investigated if such bacteria exist in terrestrial environments like soil. Here, we isolated soil bacteria that passed through a 0.1-mu m filter. The complete genome of one of the isolates was sequenced and the bacterium was identified as Hylemonella gracilis. A set of coculture assays with phylogenetically distant soil bacteria with different cell and genome sizes was performed. The coculture assays revealed that H. gracilis grows better when interacting with other soil bacteria like Paenibacillus sp. AD87 and Serratia plymuthica. Transcriptomics and metabolomics showed that H. gracilis was able to change gene expression, behavior, and biochemistry of the interacting bacteria without direct cell-cell contact. Our study indicates that in soil there are bacteria that can pass through a 0.1-mu m filter. These bacteria may have been overlooked in previous research on soil microbial communities. Such small bacteria, exemplified here by H. gracilis, can induce transcriptional and metabolomic changes in other bacteria upon their interactions in soil. In vitro, the studied interspecific interactions allowed utilization of growth substrates that could not be utilized by monocultures, suggesting that biochemical interactions between substantially different sized soil bacteria may contribute to the symbiosis of soil bacterial communities. IMPORTANCE Analysis of aquatic microbial communities revealed that parts of its diversity consist of bacteria with cell sizes of similar to 0.1 mu m. Such bacteria can show genomic reductions and metabolic dependencies with other bacteria. So far, no study investigated if such bacteria exist in terrestrial environments such as soil. Here, we show that such bacteria also exist in soil. The isolated bacteria were identified as Hylemonella gracilis. Coculture assays with phylogenetically different soil bacteria revealed that H. gracilis grows better when cocultured with other soil bacteria. Transcriptomics and metabolomics showed that H. groats was able to change gene expression, behavior, and biochemistry of the interacting bacteria without direct contact. Our study revealed that bacteria are present in soil that can pass through 0.1-mu m filters. Such bacteria may have been overlooked in previous research on soil microbial communities and may contribute to the symbiosis of soil bacterial communities.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Tyc, Olaf UNSPECIFIED orcid.org/0000-0001-9949-0975 UNSPECIFIED Kulkarni, Purva UNSPECIFIED UNSPECIFIED UNSPECIFIED Ossowicki, Adam UNSPECIFIED orcid.org/0000-0003-1903-9688 UNSPECIFIED Tracanna, Vittorio UNSPECIFIED UNSPECIFIED UNSPECIFIED Medema, Marnix H. UNSPECIFIED orcid.org/0000-0002-2191-2821 UNSPECIFIED van Baarlen, Peter UNSPECIFIED orcid.org/0000-0003-3530-5472 UNSPECIFIED van IJcken, W. F. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Verhoeven, Koen J. F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Garbeva, Paolina UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-691958
DOI:	10.1128/msystems.00574-22
Journal or Publication Title:	mSystems
Volume:	8
Number:	1
Date:	2023
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	2379-5077
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MASS-SPECTROMETRY; BACTERIAL VOLATILES; MICROBIAL DIVERSITY; GENOME; GENE; SULFUR; RHIZOSPHERE; IDENTIFICATION; BIOSYNTHESIS; ANNOTATION Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/69195