Universität zu Köln

Zaccai, Nathan R., Kadlecova, Zuzana ORCID: 0000-0001-6335-1105, Dickson, Veronica Kane, Korobchevskaya, Kseniya, Kamenicky, Jan, Kovtun, Oleksiy ORCID: 0000-0001-6374-7863, Umasankar, Perunthottathu K., Wrobel, Antoni G. ORCID: 0000-0002-6680-5587, Kaufman, Jonathan G. G., Gray, Sally R., Qu, Kun, Evans, Philip R., Fritzsche, Marco, Sroubek, Filip ORCID: 0000-0001-6835-4911, Hoening, Stefan, Briggs, John A. G., Kelly, Bernard T., Owen, David J. and Traub, Linton M. (2022). FCHO controls AP2's initiating role in endocytosis through a Ptdlns(4,5)P-2-dependent switch. Sci. Adv., 8 (17). WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE. ISSN 2375-2548

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Abstract

Clathrin-mediated endocytosis (CME) is the main mechanism by which mammalian cells control their cell surface proteome. Proper operation of the pivotal CME cargo adaptor AP2 requires membrane-localized Fer/Cip4 homology domain-only proteins (FCHO). Here, live-cell enhanced total internal reflection fluorescence-structured illumination microscopy shows that FCHO marks sites of clathrin-coated pit (CCP) initiation, which mature into uniform-sized CCPs comprising a central patch of AP2 and clathrin corralled by an FCHO/Epidermal growth factor potential receptor substrate number 15 (Eps15) ring. We dissect the network of interactions between the FCHO interdomain linker and AP2, which concentrates, orients, tethers, and partially destabilizes closed AP2 at the plasma membrane. AP2's subsequent membrane deposition drives its opening, which triggers FCHO displacement through steric competition with phosphatidylinositol 4,5-bisphosphate, clathrin, cargo, and CME accessory factors. FCHO can now relocate toward a CCP's outer edge to engage and activate further AP2s to drive CCP growth/maturation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zaccai, Nathan R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kadlecova, Zuzana UNSPECIFIED orcid.org/0000-0001-6335-1105 UNSPECIFIED Dickson, Veronica Kane UNSPECIFIED UNSPECIFIED UNSPECIFIED Korobchevskaya, Kseniya UNSPECIFIED UNSPECIFIED UNSPECIFIED Kamenicky, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Kovtun, Oleksiy UNSPECIFIED orcid.org/0000-0001-6374-7863 UNSPECIFIED Umasankar, Perunthottathu K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wrobel, Antoni G. UNSPECIFIED orcid.org/0000-0002-6680-5587 UNSPECIFIED Kaufman, Jonathan G. G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gray, Sally R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Qu, Kun UNSPECIFIED UNSPECIFIED UNSPECIFIED Evans, Philip R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Fritzsche, Marco UNSPECIFIED UNSPECIFIED UNSPECIFIED Sroubek, Filip UNSPECIFIED orcid.org/0000-0001-6835-4911 UNSPECIFIED Hoening, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Briggs, John A. G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kelly, Bernard T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Owen, David J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Traub, Linton M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-692251
DOI:	10.1126/sciadv.abn2018
Journal or Publication Title:	Sci. Adv.
Volume:	8
Number:	17
Date:	2022
Publisher:	AMER ASSOC ADVANCEMENT SCIENCE
Place of Publication:	WASHINGTON
ISSN:	2375-2548
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CLATHRIN-MEDIATED ENDOCYTOSIS; LOW-DENSITY-LIPOPROTEIN; PARTICLE CRYO-EM; CRYOELECTRON TOMOGRAPHY; STRUCTURAL EXPLANATION; MEMBRANE RECRUITMENT; ACCESSORY PROTEINS; RESOLUTION; ADAPTER; MICROSCOPY Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/69225