Universität zu Köln

Jelenik, Tomas, Kodde, Andrea, Pesta, Dominik, Phielix, Esther, Oosting, Annemarie, Rohbeck, Elisabeth, Dewidar, Bedair, Mastrototaro, Lucia, Trenkamp, Sandra, Keijer, Jaap, van der Beek, Eline M. and Roden, Michael (2022). Dietary lipid droplet structure in postnatal life improves hepatic energy and lipid metabolism in a mouse model for postnatal programming. Pharmacol. Res., 179. LONDON: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD. ISSN 1096-1186

Full text not available from this repository.

Abstract

Early-life diets may have a long-lasting impact on metabolic health. This study tested the hypothesis that an early-life diet with large, phospholipid-coated lipid droplets (Concept) induces sustained improvements of hepatic mitochondrial function and metabolism. Young C57BL/6j mice were fed Concept or control (CTRL) diet from postnatal day 15 (PN15) to PN42, followed by western style (WSD) or standard rodent diet (AIN) until PN98. Measurements comprised body composition, insulin resistance (HOMA-IR), tricarboxylic acid (TCA) cycle and beta-oxidation-related hepatic oxidative capacity using high-resolution respirometry, mitochondrial dynamics, mediators of insulin resistance (diacylglycerols, DAG) or ceramides) in subcellular compartments as well as systemic oxidative stress. Concept feeding increased TCA cycle-related respiration by 33% and mitochondrial fusion protein-1 by 65% at PN42 (both p 0.05). At PN98, CTRL, but not Concept, mice developed hyperinsulinemia (CTRL/AIN 0.22 +/- 0.44 vs. CTRL/WSD 1.49 +/- 0.53 nmol/l, p 0.05 and Concept/AIN 0.20 +/- 0.38 vs. Concept/WSD 1.00 +/- 0.29 nmol/l, n.s.) and insulin resistance after WSD (CTRL/AIN 107 +/- 23 vs. CTRL/WSD 738 +/- 284, p 0.05 and Concept/AIN 109 +/- 24 vs. Concept/WSD 524 +/- 157, n.s.). WSD-induced liver weight was 18% lower in adult Concept-fed mice and beta-oxidation-related respiration was 69% higher (p 0.05; Concept/WSD vs. Concept/AIN) along with lower plasma lipid peroxides (CTRL/AIN 4.85 +/- 0.28 vs. CTRL/WSD 5.73 +/- 0.47 mu mol/l, p 0.05 and Concept/AIN 4.49 +/- 0.31 vs. Concept/WSD 4.42 +/- 0.33 mu mol/l, n.s.) and were in part protected from WSD-induced increase in hepatic cytosolic DAG C16:0/C18:1. Early-life feeding of Concept partly protected from WSD-induced insulin resistance and systemic oxidative stress, potentially via changes in specific DAG and mitochondrial function, highlighting the role of early life diets on metabolic health later in life.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Jelenik, Tomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Kodde, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Pesta, Dominik UNSPECIFIED UNSPECIFIED UNSPECIFIED Phielix, Esther UNSPECIFIED UNSPECIFIED UNSPECIFIED Oosting, Annemarie UNSPECIFIED UNSPECIFIED UNSPECIFIED Rohbeck, Elisabeth UNSPECIFIED UNSPECIFIED UNSPECIFIED Dewidar, Bedair UNSPECIFIED UNSPECIFIED UNSPECIFIED Mastrototaro, Lucia UNSPECIFIED UNSPECIFIED UNSPECIFIED Trenkamp, Sandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Keijer, Jaap UNSPECIFIED UNSPECIFIED UNSPECIFIED van der Beek, Eline M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Roden, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-692336
DOI:	10.1016/j.phrs.2022.106193
Journal or Publication Title:	Pharmacol. Res.
Volume:	179
Date:	2022
Publisher:	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Place of Publication:	LONDON
ISSN:	1096-1186
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NONALCOHOLIC FATTY LIVER; INSULIN-RESISTANCE; MITOCHONDRIAL-FUNCTION; PERMEABILIZED FIBERS; HOMEOSTASIS MODEL; MATERNAL OBESITY; GLOBULE-MEMBRANE; OXPHOS PROTOCOLS; GENE-EXPRESSION; ADIPOSE-TISSUE Multiple languages Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/69233