Universität zu Köln

Chew, Elaine G. Y., Lim, Tze Chiun, Leong, Meng Fatt, Liu, Xingliang, Sia, Yee Yen, Leong, See Ting, Yan-Jiang, Benjamin C., Stoecklin, Celine, Borhan, Rosa, Heilmann-Heimbach, Stefanie ORCID: 0000-0003-1057-465X, Nothen, Markus M., Viasnoff, Virgile, Shyh-Chang, Ng ORCID: 0000-0003-3138-9525, Wan, Andrew C. A., Philpott, Michael P. and Hillmer, Axel M. (2022). Observations that suggest a contribution of altered dermal papilla mitochondrial function to androgenetic alopecia. Exp. Dermatol., 31 (6). S. 906 - 918. HOBOKEN: WILEY. ISSN 1600-0625

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Abstract

Androgenetic alopecia (AGA) is a prevalent hair loss condition in males that develops due to the influence of androgens and genetic predisposition. With the aim of elucidating genes involved in AGA pathogenesis, we modelled AGA with three-dimensional culture of keratinocyte-surrounded dermal papilla (DP) cells. We co-cultured immortalised balding and non-balding human DP cells (DPCs) derived from male AGA patients with epidermal keratinocyte (NHEK) using multi-interfacial polyelectrolyte complexation technique. We observed up-regulated mitochondria-related gene expression in balding compared with non-balding DP aggregates which indicated altered mitochondria metabolism. Further observation of significantly reduced electron transport chain complex activity (complexes I, IV and V), ATP levels and ability to uptake metabolites for ATP generation demonstrated compromised mitochondria function in balding DPC. Balding DP was also found to be under significantly higher oxidative stress than non-balding DP. Our experiments suggest that application of antioxidants lowers oxidative stress levels and improves metabolite uptake in balding DPC. We postulate that the observed up-regulation of mitochondria-related genes in balding DP aggregates resulted from an over-compensatory effort to rescue decreased mitochondrial function in balding DP through the attempted production of new functional mitochondria. In all, our three-dimensional co-culturing revealed mitochondrial dysfunction in balding DPC, suggesting a metabolic component in the aetiology of AGA.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Chew, Elaine G. Y. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lim, Tze Chiun UNSPECIFIED UNSPECIFIED UNSPECIFIED Leong, Meng Fatt UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Xingliang UNSPECIFIED UNSPECIFIED UNSPECIFIED Sia, Yee Yen UNSPECIFIED UNSPECIFIED UNSPECIFIED Leong, See Ting UNSPECIFIED UNSPECIFIED UNSPECIFIED Yan-Jiang, Benjamin C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Stoecklin, Celine UNSPECIFIED UNSPECIFIED UNSPECIFIED Borhan, Rosa UNSPECIFIED UNSPECIFIED UNSPECIFIED Heilmann-Heimbach, Stefanie UNSPECIFIED orcid.org/0000-0003-1057-465X UNSPECIFIED Nothen, Markus M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Viasnoff, Virgile UNSPECIFIED UNSPECIFIED UNSPECIFIED Shyh-Chang, Ng UNSPECIFIED orcid.org/0000-0003-3138-9525 UNSPECIFIED Wan, Andrew C. A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Philpott, Michael P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hillmer, Axel M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-692660
DOI:	10.1111/exd.14536
Journal or Publication Title:	Exp. Dermatol.
Volume:	31
Number:	6
Page Range:	S. 906 - 918
Date:	2022
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1600-0625
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MALE-PATTERN BALDNESS; OUTER ROOT SHEATH; MAMMALIAN PEROXIREDOXIN; COACTIVATOR HIC-5/ARA55; LIGHT THERAPY; DOUBLE-BLIND; HAIR-GROWTH; STEM-CELL; RECEPTOR; IDENTIFICATION Multiple languages Dermatology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/69266