Universität zu Köln

Domdey, M., Kluth, M. A., Masslo, C., Ganss, C., Frank, M. H., Frank, N. Y., Coroneo, M. T., Cursiefen, C. and Notara, M. (2022). Consecutive dosing of UVB irradiation induces loss of ABCB5 expression and activation of EMT and fibrosis proteins in limbal epithelial cells similar to pterygium epithelium. Stem Cell Res., 64. AMSTERDAM: ELSEVIER. ISSN 1876-7753

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Abstract

Pterygium pathogenesis is often attributed to a population of altered limbal stem cells, which initiate corneal invasion and drive the hyperproliferation and fibrosis associated with the disease. These cells are thought to undergo epithelial to mesenchymal transition (EMT) and to contribute to subepithelial stromal fibrosis. In this study, the presence of the novel limbal stem cell marker ABCB5 in clusters of basal epithelial pterygium cells coexpressing with P63 alpha and P40 is reported. ABCB5-positive pterygium cells also express EMT-associated fibrosis markers including vimentin and alpha-SMA while their beta-catenin expression is reduced. By using a novel in vitro model of two-dose UV-induced EMT activation on limbal epithelial cells, we could observe the dysregulation of EMT-related proteins including an increase of vimentin and alpha-SMA as well as downregulation of beta-catenin in epithelial cells correlating to downregulation of ABCB5. The sequential irradiation of limbal fibroblasts also induced an increase in vimentin and alpha-SMA. Taken together, these data demonstrate for the first time the expression of ABCB5 in pterygium stem cell activity and EMT-related events while the involvement of limbal stem cells in pterygium pathogenesis is exhibited via sequential irradiation of limbal epithelial cells. The later in vitro approach can be used to further study the involvement of limbal epithelium UV-induced EMT in pterygium pathogenesis and help identify novel treatments against pterygium growth and recurrence.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Domdey, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kluth, M. A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Masslo, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ganss, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Frank, M. H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Frank, N. Y. UNSPECIFIED UNSPECIFIED UNSPECIFIED Coroneo, M. T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cursiefen, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Notara, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-693167
DOI:	10.1016/j.scr.2022.102936
Journal or Publication Title:	Stem Cell Res.
Volume:	64
Date:	2022
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1876-7753
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MESENCHYMAL TRANSITION; TRANSPORTER ABCB5; P63; ULTRAVIOLET; PATHOGENESIS; INVASION; DAMAGE; MODEL; LEADS Multiple languages Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/69316