Universität zu Köln

Noster, Janina ORCID: 0000-0002-8802-6605, Koeppel, Martin B., Desnos-Olivier, Marie, Aigner, Maria, Bader, Oliver ORCID: 0000-0002-1534-440X, Dichtl, Karl ORCID: 0000-0001-5032-8870, Goettig, Stephan, Haas, Andrea, Kurzai, Oliver ORCID: 0000-0002-7277-2646, Pranada, Arthur B., Stelzer, Yvonne, Walther, Grit and Hamprecht, Axel (2022). Bloodstream Infections Caused by Magnusiomyces capitatus and Magnusiomyces clavatus: Epidemiological, Clinical, and Microbiological Features of Two Emerging Yeast Species. Antimicrob. Agents Chemother., 66 (2). WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-6596

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Abstract

Magnusiomyces clavatus and Magnusiomyces capitatus are emerging yeasts with intrinsic resistance to many commonly used antifungal agents. Identification is difficult, and determination of susceptibility patterns with commercial and reference methods is equally challenging. For this reason, few data on invasive infections by Magnusiomyces spp. are available. Our objectives were to determine the epidemiology and susceptibility of Magnusiomyces isolates from bloodstream infections (BSI) isolated in Germany and Austria from 2001 to 2020. In seven institutions, a total of 34 Magnusiomyces BSI were identified. Identification was done by internal transcribed spacer (ITS) sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Antifungal susceptibility was determined by EUCAST broth microdilution and gradient tests. Of the 34 isolates, M. clavatus was more common (n = 24) than M. capitatus (n = 10). BSI by Magnusiomyces spp. were more common in men (62%) and mostly occurred in patients with hemato-oncological malignancies (79%). The highest in vitro antifungal activity against M. clavatus/M. capitatus was observed for voriconazole (MIC50, 0.03/0.125 mg/L), followed by posaconazole (MIC50, 0.125/025 mg/L). M. clavatus isolates showed overall lower MICs than M. capitatus. With the exception of amphotericin B, low essential agreement between gradient test and microdilution was recorded for all antifungals (0 to 70%). Both species showed distinct morphologic traits on ChromAgar Orientation medium and Columbia blood agar, which can be used for differentiation if no MALDI-TOF MS or molecular identification is available. In conclusion, most BSI were caused by M. clavatus. The lowest MICs were recorded for voriconazole. Gradient tests demonstrated unacceptably low agreement and should preferably not be used for susceptibility testing of Magnusiomyces spp.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Noster, Janina UNSPECIFIED orcid.org/0000-0002-8802-6605 UNSPECIFIED Koeppel, Martin B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Desnos-Olivier, Marie UNSPECIFIED UNSPECIFIED UNSPECIFIED Aigner, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Bader, Oliver UNSPECIFIED orcid.org/0000-0002-1534-440X UNSPECIFIED Dichtl, Karl UNSPECIFIED orcid.org/0000-0001-5032-8870 UNSPECIFIED Goettig, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Haas, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Kurzai, Oliver UNSPECIFIED orcid.org/0000-0002-7277-2646 UNSPECIFIED Pranada, Arthur B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Stelzer, Yvonne UNSPECIFIED UNSPECIFIED UNSPECIFIED Walther, Grit UNSPECIFIED UNSPECIFIED UNSPECIFIED Hamprecht, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-693501
DOI:	10.1128/aac.01834-21
Journal or Publication Title:	Antimicrob. Agents Chemother.
Volume:	66
Number:	2
Date:	2022
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-6596
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DESORPTION IONIZATION-TIME; INVASIVE INFECTIONS; SAPROCHAETE-CLAVATA; HEMATOLOGICAL MALIGNANCIES; GEOTRICHUM-CAPITATUM; DISK DIFFUSION; IDENTIFICATION; VORICONAZOLE; FLUCONAZOLE; CANDIDA Multiple languages Microbiology; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/69350