Universität zu Köln

Peltier, Manon ORCID: 0009-0008-3832-4111, Raspa, Marcello, Putti, Sabrina, Paoletti, Renata, Scavizzi, Ferdinando and Mahabir, Esther ORCID: 0000-0002-9573-5127 (2025). Cryopreservation at −80 °C impacts sperm integrity and fertility in a mouse strain-dependent manner. Theriogenology, 245. pp. 1-11. Elsevier. ISSN 0093691X

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Abstract

[Artikel-Nr.: 117523] We previously developed a simple method to cryopreserve and maintain B6N spermatozoa at −80 °C, instead of using liquid nitrogen (LN2), for up to one year without detrimental effects on fertility. The goal of the present study was to test the efficacy of this method in six different mouse strains (129/SvImJCnrm; 129, C57BL/6JCnrm; B6J, C57BL/6NTacCnrm; B6N, BALB/cByJCnrm; BALB/c, Crl:CD1(ICR); CD-1, and FVBN/JCnrm; FVB). At different time points up to one year, we evaluated the integrity and the fertility of the spermatozoa cryopreserved and stored at −80 °C in comparison to the classical LN2 method. After 1 year, no differences in the in vitro fertilisation (IVF) rate and in the number of dead spermatozoa were observed in 129, B6N, CD-1 and FVB strains. In contrast, spermatozoa from B6J and BALB/c cryopreserved at −80 °C showed a significant reduction in the IVF rate, an increased number of dead spermatozoa and increased morphological abnormalities after 3 months (BALB/c) and 6 months (B6J). In all strains, the total sperm motility decreased after 1 month at −80 °C and increased ultrastructural damage was found in the −80 °C group. The present results indicate that spermatozoa from 129, B6N, CD-1 and FVB can be cryopreserved and stored at −80 °C for at least one year without impacting their fertility. However, spermatozoa from B6J and BALB/c are the most sensitive to temperature. Therefore, cryopreservation and storage of 129, B6N, CD-1 and FVB strains at −80 °C for up to one year is feasible, thereby circumventing the use of LN2.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Peltier, Manon UNSPECIFIED https://orcid.org/0009-0008-3832-4111 UNSPECIFIED Raspa, Marcello UNSPECIFIED UNSPECIFIED UNSPECIFIED Putti, Sabrina UNSPECIFIED UNSPECIFIED UNSPECIFIED Paoletti, Renata UNSPECIFIED UNSPECIFIED UNSPECIFIED Scavizzi, Ferdinando UNSPECIFIED UNSPECIFIED UNSPECIFIED Mahabir, Esther UNSPECIFIED https://orcid.org/0000-0002-9573-5127 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-805746
Identification Number:	10.1016/j.theriogenology.2025.117523
Journal or Publication Title:	Theriogenology
Volume:	245
Page Range:	pp. 1-11
Number of Pages:	11
Date:	October 2025
Publisher:	Elsevier
ISSN:	0093691X
Language:	English
Faculty:	Faculty of Medicine
Divisions:	Zentrum für Molekulare Medizin
Subjects:	Medical sciences Medicine
Uncontrolled Keywords:	Keywords Language sperm cryodamage ; Cryopreservation ; In vitro fertilisation ; Mouse spermatozoa ; -80 ◦C freezer English
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/80574