Universität zu Köln

Hunold, Pascal ORCID: 0000-0002-7087-5908, Pizzolato, Giulia ORCID: 0000-0002-0776-456X, Heramvand, Nadia, Kaiser, Laura ORCID: 0000-0002-3212-576X, Barbiera, Giulia, van Ray, Olivia ORCID: 0000-0002-1449-0018, Thomas, Roman ORCID: 0000-0001-9132-4876, George, Julie ORCID: 0000-0002-4272-3683, Peifer, Martin ORCID: 0000-0002-5243-5503 and Hänsel-Hertsch, Robert ORCID: 0000-0002-2835-4471 (2025). DynaTag for efficient mapping of transcription factors in low-input samples and at single-cell resolution. Nature Communications, 16 (1). pp. 1-15. Springer Nature. ISSN 2041-1723

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Abstract

[Artikel-Nr.: 6585] Systematic discovery of transcription factor (TF) landscapes in low-input samples and at single cell level is a major challenge in the fields of molecular biology, genetics, and epigenetics. Here, we present cleavage under Dyna mic targets and Tag mentation (DynaTag), enabling robust mapping of TF-DNA interactions using a physiological salt solution during sample preparation. DynaTag uncovers occupancy alterations for 15 TFs in stem cell and cancer tissue models. We highlight changes in TF-DNA binding for NANOG, MYC, and OCT4, during stem-cell differentiation, at both bulk and single-cell resolutions. DynaTag surpasses CUT&RUN and ChIP-seq in signal-to-background ratio and resolution. Furthermore, using tumours of a small cell lung cancer model derived from a single female donor, DynaTag reveals increased chromatin occupancy of FOXA1, MYC, and the mutant p53 R248Q at enriched gene pathways (e.g. epithelial-mesenchymal transition), following chemotherapy treatment. Collectively, we believe that DynaTag represents a significant technological advancement, facilitating precise characterization of TF landscapes across diverse biological systems and complex models.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Hunold, Pascal UNSPECIFIED https://orcid.org/0000-0002-7087-5908 UNSPECIFIED Pizzolato, Giulia UNSPECIFIED https://orcid.org/0000-0002-0776-456X UNSPECIFIED Heramvand, Nadia UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaiser, Laura UNSPECIFIED https://orcid.org/0000-0002-3212-576X UNSPECIFIED Barbiera, Giulia UNSPECIFIED UNSPECIFIED UNSPECIFIED van Ray, Olivia UNSPECIFIED https://orcid.org/0000-0002-1449-0018 UNSPECIFIED Thomas, Roman UNSPECIFIED https://orcid.org/0000-0001-9132-4876 UNSPECIFIED George, Julie UNSPECIFIED https://orcid.org/0000-0002-4272-3683 UNSPECIFIED Peifer, Martin UNSPECIFIED https://orcid.org/0000-0002-5243-5503 UNSPECIFIED Hänsel-Hertsch, Robert UNSPECIFIED https://orcid.org/0000-0002-2835-4471 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-806375
Identification Number:	10.1038/s41467-025-61797-9
Journal or Publication Title:	Nature Communications
Volume:	16
Number:	1
Page Range:	pp. 1-15
Number of Pages:	15
Date:	28 July 2025
Publisher:	Springer Nature
ISSN:	2041-1723
Language:	English
Faculty:	Faculty of Medicine
Divisions:	Faculty of Medicine > Hals-Nasen-Ohrenheilkunde > Klinik und Poliklinik für Hals-, Nasen- und Ohrenheilkunde Faculty of Medicine > Humangenetik > Institut für Humangenetik Faculty of Medicine > Pathologie und Neuropathologie > Institut für Pathologie Faculty of Medicine > Weitere > Translationale Genomik Zentrum für Molekulare Medizin
Subjects:	Medical sciences Medicine
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/80637