Universität zu Köln

Chen, Shiyi, Hassan, Nourhan ORCID: 0000-0003-3189-8063, Kopp, Alexander, Eufrásio-da-Silva, Tatiane, Arfaoui, Jihene ORCID: 0000-0003-2158-1185, Isella, Benedetta, Aytuna, Ziyaad, Barnowski, Philipp, Sengle, Gerhard, Dolatshahi-Pirouz, Alireza, Kröger, Nadja and Maleki, Hajar Homa ORCID: 0000-0002-2813-4700 (2025). Theragenerative injectable bone-adhesive hydrogels for combined photothermal osteosarcoma therapy and bone repair. Biomaterials Science, 13 (13). pp. 3544-3560. Royal Society of Chemistry. ISSN 2047-4830

PDF d5bm00559k.pdf Bereitstellung unter der CC-Lizenz: Creative Commons Attribution. Download (5MB)

Abstract

Injectable hydrogels with self-healing properties, tissue adhesion, biocompatibility, and cancer therapeutic capabilities offer a promising solution for addressing bone loss and residual tumor cells following surgical resection of osteosarcoma. In this study, injectable adhesive hybrid hydrogels were developed using natural silk-derived proteins, silk fibroin (SF), and silk sericin (SS). The sericin was surface functionalized with dopamine (DOPA) forming SSDOPA, while the silk fibroin was enzymatically oxidized (forming SFO) to introduce abundant catechol moieties on the polymer chains. These modifications enabled hydrogelation and self-assembly in the presence of copper ions (Cu 2+ ) and tannic acid (TA), creating an SFO-SSDopa-Cu 2+ -TA hydrogel inspired by the mussel adhesion mechanism. The dynamic metal-catechol coordination bonds, along with other covalent and non-covalent interactions in the gel network, imparted excellent shear-thinning properties with 3D printability, injectability, self-healing (72.27 ± 9.35% after 6 cyclic), making it suitable for minimally invasive surgeries and targeted delivery applications. Additionally, the developed adhesive hydrogel demonstrated strong adhesiveness (664.03 ± 15.87 kPa and 854.15 ± 12.90 kPa on Gel- and Hap-based substrates respectively), showing excellent bonding performance to natural bone and tissue. Its black coloration enabled efficient absorption of near-infrared (NIR) light (reach 45–48 °C), facilitating the eradication of almost 60% osteosarcoma cells through photothermal therapy within 20 minutes of hydrogel irradiation with laser. Moreover, the developed SFO-SSDopa-Cu 2+ -TA hydrogels promoted the proliferation and migration of pre-osteoblast cells, confirming their excellent biocompatibility. Coupled with good biodegradability, these hydrogels demonstrate significant potential as theragenerative materials for minimally invasive osteosarcoma treatment, providing a clinically translatable solution for repairing bone affected by the disease.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Chen, Shiyi UNSPECIFIED UNSPECIFIED UNSPECIFIED Hassan, Nourhan UNSPECIFIED https://orcid.org/0000-0003-3189-8063 UNSPECIFIED Kopp, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Eufrásio-da-Silva, Tatiane UNSPECIFIED UNSPECIFIED UNSPECIFIED Arfaoui, Jihene UNSPECIFIED https://orcid.org/0000-0003-2158-1185 UNSPECIFIED Isella, Benedetta UNSPECIFIED UNSPECIFIED UNSPECIFIED Aytuna, Ziyaad UNSPECIFIED UNSPECIFIED UNSPECIFIED Barnowski, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED Sengle, Gerhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Dolatshahi-Pirouz, Alireza UNSPECIFIED UNSPECIFIED UNSPECIFIED Kröger, Nadja UNSPECIFIED UNSPECIFIED UNSPECIFIED Maleki, Hajar Homa UNSPECIFIED https://orcid.org/0000-0002-2813-4700 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-811671
Identification Number:	10.1039/D5BM00559K
Journal or Publication Title:	Biomaterials Science
Volume:	13
Number:	13
Page Range:	pp. 3544-3560
Number of Pages:	17
Date:	2025
Publisher:	Royal Society of Chemistry
ISSN:	2047-4830
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences Faculty of Medicine
Divisions:	CECAD - Cluster of Excellence Cellular Stress Responses in Aging-Associated Diseases Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Inorganic Chemistry Faculty of Medicine > Biochemie > Zentrum für Biochemie Faculty of Medicine > Kinder- und Jugendmedizin > Klinik und Poliklinik für Kinder- und Jugendmedizin Zentrum für Molekulare Medizin
Subjects:	Medical sciences Medicine
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/81167