Universität zu Köln

Hose, Jeremai ORCID: 0000-0003-4085-2219, Stach, Paul ORCID: 0009-0008-8173-196X, Reda, Sara ORCID: 0009-0006-2650-4356, Juebner, Martin ORCID: 0000-0002-5145-4884, Thevis, Mario ORCID: 0000-0002-1535-6451 and Andresen-Streichert, Hilke (2026). Device does matter! Comparison of PEth 16:0/18:1 and PEth 16:0/18:2 blood concentrations determined from three dried blood spot sampling devices. Journal of Pharmaceutical and Biomedical Analysis, 271. pp. 1-7. Elsevier. ISSN 0731-7085

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Abstract

[Artikel-Nr.: 117325] The biomarker phosphatidylethanol (PEth) can be determined in whole blood to distinguish between alcohol abstinence and alcohol consumption. Routine analysis is commonly performed using dried blood spots (DBS), as PEth is considered stable for extended time periods in this matrix. However, the coexistence of many DBS devices, coupled with insufficient data regarding their comparability of results, pose a major challenge in forensic and clinical practice. We therefore analysed and compared blood samples using three DBS devices commonly employed for PEth testing (Whatman™ filter paper, Mitra® and Capitainer®B Vanadate). PEth 16:0/18:1 and 16:0/18:2 were extracted from DBS using a water/propan-2-ol mixture and subsequent liquid-liquid-extraction using n-hexane. Analysis was carried out via LC-MS/MS. Ethanol and acetaldehyde concentrations were determined in each blood sample via GC-FID. A total of 100 blood samples were analysed, of which 76 were found to be positive for PEth. These included 24 cases with PEth concentrations < 20 ng/mL, 43 cases with concentrations ≥ 20 ng/mL to < 200 ng/mL and nine cases with concentrations ≥ 200 ng/mL. The Mitra® and Capitainer®B Vanadate systems resulted, on average, in higher PEth concentrations compared to Whatman™ filter paper. The percentage deviations ranged from −8–116 % for PEth 16:0/18:1 and from −14–264 % for PEth 16:0/18:2. Ethanol and acetaldehyde above physiological concentrations (1.42 g/L and 0.727 mg/L, respectively) were observed in one case only. While DBS are a reliable method for PEth analysis, the choice of device can influence results and potentially impact interpretations of alcohol use and abstinence.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Hose, Jeremai UNSPECIFIED https://orcid.org/0000-0003-4085-2219 UNSPECIFIED Stach, Paul UNSPECIFIED https://orcid.org/0009-0008-8173-196X UNSPECIFIED Reda, Sara UNSPECIFIED https://orcid.org/0009-0006-2650-4356 UNSPECIFIED Juebner, Martin UNSPECIFIED https://orcid.org/0000-0002-5145-4884 UNSPECIFIED Thevis, Mario UNSPECIFIED https://orcid.org/0000-0002-1535-6451 UNSPECIFIED Andresen-Streichert, Hilke UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-812863
Identification Number:	10.1016/j.jpba.2025.117325
Journal or Publication Title:	Journal of Pharmaceutical and Biomedical Analysis
Volume:	271
Page Range:	pp. 1-7
Number of Pages:	7
Date:	15 April 2026
Publisher:	Elsevier
ISSN:	0731-7085
Language:	English
Faculty:	Faculty of Medicine
Divisions:	Faculty of Medicine > Rechtsmedizin > Institut für Rechtsmedizin Faculty of Medicine > Transfusionsmedizin > Zentrale Dienstleistungseinrichtung für Transfusionsmedizin
Subjects:	Medical sciences Medicine
Uncontrolled Keywords:	Keywords Language Alcohol biomarker ; Alternative matrices ; Cut-off ; Red blood cell transfusion English
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/81286