Universität zu Köln

Angermann, Cecile ORCID: 0000-0002-1051-5679, Heinemann, Björn ORCID: 0000-0002-3962-4174, Nogueira, Bianca Bueno, Mai, Hans‐Jörg, Bauer, Petra ORCID: 0000-0002-0404-4532 and Hildebrandt, Tatjana M. ORCID: 0000-0002-3562-5241 (2025). Balancing nutrient remobilization and photosynthesis: proteomic insights into the dual role of lupin cotyledons after germination. The Plant Journal, 123 (2). pp. 1-18. Wiley. ISSN 0960-7412

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Abstract

[Artikel-Nr.: e70357] Significance Statement: This study uncovers how white lupin cotyledons balance nutrient storage and photosynthesis during early seedling development, ensuring sustained nitrogen and mineral supply under nutrient‐deficient conditions. By detailing the proteome reorganization and consequences of premature cotyledon loss, it identifies key mechanisms supporting seedling resilience and adaptation to low‐fertility soils. - Efficient nutrient mobilization from seed storage tissues is essential for seedling establishment, particularly in legumes such as Lupinus albus (white lupin), which thrive in nutrient-poor soils. This study investigates the role of cotyledons in nitrogen (N) and mineral remobilization after germination during their transition from storage organs to photosynthetically active tissues, including the metabolic challenges posed by the coexistence of these two functions in epigeal germination. We cultivated white lupin seedlings under nitrogen-deficient conditions, analyzing cotyledon composition and function over 28 days. Our results indicate that 60% of cotyledon-stored proteins are degraded within the first 8 days, with free amino acids transiently accumulating before being redistributed to support growth. The progressive depletion of cotyledon reserves was accompanied by structural and metabolic changes, including an increase in photosynthetic proteins. However, cotyledon photosynthetic capacity remained lower than that of true leaves, suggesting a transient role in energy metabolism. The loss of cotyledons before day 12 significantly impaired seedling development, emphasizing their critical contribution to nitrogen, phosphate, and micronutrient supply during early growth. Comparative proteomic analysis revealed dynamic shifts in nutrient transport, amino acid metabolism, and stress response pathways following cotyledon removal. These findings underscore the significance of cotyledon nutrient remobilization in legume adaptation to low-fertility soils and highlight potential targets for breeding strategies aimed at improving nutrient use efficiency. By optimizing cotyledon nutrient composition and function, future breeding efforts could enhance seedling vigor, reduce fertilizer dependency, and improve the nutritional value of lupin-based foods.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Angermann, Cecile UNSPECIFIED https://orcid.org/0000-0002-1051-5679 UNSPECIFIED Heinemann, Björn UNSPECIFIED https://orcid.org/0000-0002-3962-4174 UNSPECIFIED Nogueira, Bianca Bueno UNSPECIFIED UNSPECIFIED UNSPECIFIED Mai, Hans‐Jörg UNSPECIFIED UNSPECIFIED UNSPECIFIED Bauer, Petra UNSPECIFIED https://orcid.org/0000-0002-0404-4532 UNSPECIFIED Hildebrandt, Tatjana M. UNSPECIFIED https://orcid.org/0000-0002-3562-5241 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-813080
Identification Number:	10.1111/tpj.70357
Journal or Publication Title:	The Plant Journal
Volume:	123
Number:	2
Page Range:	pp. 1-18
Number of Pages:	18
Date:	21 July 2025
Publisher:	Wiley
ISSN:	0960-7412
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	CEPLAS - Cluster of Excellence on Plant Sciences
Subjects:	Life sciences
Uncontrolled Keywords:	Keywords Language amino acid metabolism ; Lupinus albus ; mineral nutrients ; nitrogen storage ; resource remobilization English
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/81308