Universität zu Köln

Müller, Leonie ORCID: 0009-0007-6561-1565, Salman, Sirin ORCID: 0009-0004-6567-701X and Hoppe, Thorsten ORCID: 0000-0002-4734-9352 (2024). Chemical cross-linking to study protein self-assembly in cellulo. STAR Protocols, 5 (2). pp. 1-16. Elsevier. ISSN 2666-1667

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Abstract

[Artikel-Nr.: 103032] Many proteins self-assemble into dimers and higher-order oligomers. Therefore, the goal of this protocol is to characterize the conformational states of an endog- enous protein of interest. Here, we present a protocol for assessing protein self-assembly in cell lysates using chemical cross-linking. We describe steps for chemical cross-linking with recombinant proteins as well as steps for cell culture and cell lysate preparation, chemical cross-linking, SDS-PAGE, and western blot- ting for the detection of endogenous proteins.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Müller, Leonie UNSPECIFIED https://orcid.org/0009-0007-6561-1565 UNSPECIFIED Salman, Sirin UNSPECIFIED https://orcid.org/0009-0004-6567-701X UNSPECIFIED Hoppe, Thorsten UNSPECIFIED https://orcid.org/0000-0002-4734-9352 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-813750
Identification Number:	10.1016/j.xpro.2024.103032
Journal or Publication Title:	STAR Protocols
Volume:	5
Number:	2
Page Range:	pp. 1-16
Number of Pages:	16
Date:	21 June 2024
Publisher:	Elsevier
ISSN:	2666-1667
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences Faculty of Medicine
Divisions:	CECAD - Cluster of Excellence Cellular Stress Responses in Aging-Associated Diseases Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics Zentrum für Molekulare Medizin
Subjects:	Life sciences Medical sciences Medicine
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/81375