Albus, Alexandra ORCID: 0000-0002-4897-6972, Kreer, Christoph ORCID: 0000-0002-9140-9850, Klein, Florian ORCID: 0000-0003-1376-1792, Rybniker, Jan ORCID: 0000-0001-8351-2690 and Simonis, Alexander ORCID: 0000-0003-2945-9897 (2024). Protocol for developing Pseudomonas aeruginosa type III secretion system-neutralizing monoclonal antibodies from human B cells. STAR Protocols, 5 (4). pp. 1-24. Elsevier. ISSN 2666-1667

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Identification Number:10.1016/j.xpro.2024.103440

Abstract

[Artikel-Nr.: 103440] Monoclonal antibodies (mAbs) targeting bacterial virulence factors may represent promising therapeutics in the fight against severe bacterial infections. Here, we present an approach for developing human-derived antibodies targeting the type III secretion system (T3SS) of Pseudomonas aeruginosa (PA) by neutralizing the function of the T3SS-tip protein PcrV. The protocol involves identifying individuals with protective antibodies, isolating PcrV-specific B cells from these individuals, and producing and testing anti-PcrV mAbs derived from single B cells.

Item Type: Article
Creators:
Creators
Email
ORCID
ORCID Put Code
Albus, Alexandra
UNSPECIFIED
https://orcid.org/0000-0002-4897-6972
UNSPECIFIED
Kreer, Christoph
UNSPECIFIED
https://orcid.org/0000-0002-9140-9850
UNSPECIFIED
Klein, Florian
UNSPECIFIED
https://orcid.org/0000-0003-1376-1792
UNSPECIFIED
Rybniker, Jan
UNSPECIFIED
https://orcid.org/0000-0001-8351-2690
UNSPECIFIED
Simonis, Alexander
UNSPECIFIED
https://orcid.org/0000-0003-2945-9897
UNSPECIFIED
URN: urn:nbn:de:hbz:38-814462
Identification Number: 10.1016/j.xpro.2024.103440
Journal or Publication Title: STAR Protocols
Volume: 5
Number: 4
Page Range: pp. 1-24
Number of Pages: 14
Date: December 2024
Publisher: Elsevier
ISSN: 2666-1667
Language: English
Faculty: Faculty of Medicine
Divisions: Faculty of Medicine > Innere Medizin > Klinik I für Innere Medizin - Hämatologie und Onkologie
Faculty of Medicine > Virologie > Institut für Virologie
Zentrum für Molekulare Medizin
Subjects: Medical sciences Medicine
['eprint_fieldname_oa_funders' not defined]: Publikationsfonds UzK
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/81446

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