Universität zu Köln

Schiavinato, Alvise ORCID: 0000-0002-8969-4074, Marcous, Fady ORCID: 0009-0006-2209-7773, Zuk, Alexandra V., Keene, Douglas R., Tufa, Sara F., Mosquera, Laura M., Zigrino, Paola ORCID: 0000-0002-7470-0064, Mauch, Cornelia ORCID: 0000-0002-3989-0536, Eckes, Beate ORCID: 0000-0002-0936-0702, Francois, Katrien, De Backer, Julie, Hunzelmann, Nicolas, Moinzadeh, Pia ORCID: 0000-0002-8784-8615, Krieg, Thomas ORCID: 0000-0001-5616-8476, Callewaert, Bert and Sengle, Gerhard ORCID: 0000-0003-3932-1242 (2024). New insights into the structural role of EMILINs within the human skin microenvironment. Scientific Reports, 14 (1). pp. 1-15. Springer Nature. ISSN 2045-2322

PDF s41598-024-81509-5.pdf Bereitstellung unter der CC-Lizenz: Creative Commons Attribution. Download (5MB)

Abstract

[Artikel-Nr.: 30345] Supramolecular extracellular matrix (ECM) networks play an essential role in skin architecture and function. Elastin microfibril interface-located proteins (EMILINs) comprise a family of three extracellular glycoproteins that serve as essential structural components of the elastin/fibrillin microfibril network, and exert crucial functions in cellular signaling. Little is known about the structural nature of EMILIN networks in skin. We therefore investigated the spatiotemporal localization of EMILIN-1, -2, -3 in human skin induced by aging, UV-exposure, fibrosis, and connective tissue disorder. Confocal immunofluorescence and immunogold electron microscopy analysis identified all EMILINs as components of elastic fibers and elastin-free oxytalan fibers inserted into the basement membrane (BM). Further, our ultrastructural analysis demonstrates cellular contacts of dermally localized EMILIN-1 positive fibers across the BM with the surface of basal keratinocytes. Analysis of skin biopsies and fibroblast cultures from fibrillin-1 deficient Marfan patients revealed that EMILINs require intact fibrillin-1 as deposition scaffold. In patients with scleroderma and the bleomycin-induced murine fibrosis model EMILIN-2 was upregulated. EMILIN-3 localizes to the tips of candelabra-like oxytalan fibers, and to specialized BMs engulfing hair follicles and sebaceous glands. Our data identify EMILINs as important markers to monitor rearrangements of the dermal ECM architecture induced by aging and pathological conditions.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Schiavinato, Alvise UNSPECIFIED https://orcid.org/0000-0002-8969-4074 UNSPECIFIED Marcous, Fady UNSPECIFIED https://orcid.org/0009-0006-2209-7773 UNSPECIFIED Zuk, Alexandra V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Keene, Douglas R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Tufa, Sara F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mosquera, Laura M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zigrino, Paola UNSPECIFIED https://orcid.org/0000-0002-7470-0064 UNSPECIFIED Mauch, Cornelia UNSPECIFIED https://orcid.org/0000-0002-3989-0536 UNSPECIFIED Eckes, Beate UNSPECIFIED https://orcid.org/0000-0002-0936-0702 UNSPECIFIED Francois, Katrien UNSPECIFIED UNSPECIFIED UNSPECIFIED De Backer, Julie UNSPECIFIED UNSPECIFIED UNSPECIFIED Hunzelmann, Nicolas UNSPECIFIED UNSPECIFIED UNSPECIFIED Moinzadeh, Pia UNSPECIFIED https://orcid.org/0000-0002-8784-8615 UNSPECIFIED Krieg, Thomas UNSPECIFIED https://orcid.org/0000-0001-5616-8476 UNSPECIFIED Callewaert, Bert UNSPECIFIED UNSPECIFIED UNSPECIFIED Sengle, Gerhard UNSPECIFIED https://orcid.org/0000-0003-3932-1242 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-816099
Identification Number:	10.1038/s41598-024-81509-5
Journal or Publication Title:	Scientific Reports
Volume:	14
Number:	1
Page Range:	pp. 1-15
Number of Pages:	15
Date:	5 December 2024
Publisher:	Springer Nature
ISSN:	2045-2322
Language:	English
Faculty:	Central Institutions / Interdisciplinary Research Centers Collaborative Research Centers Faculty of Medicine
Divisions:	CECAD - Cluster of Excellence Cellular Stress Responses in Aging-Associated Diseases Faculty of Medicine > Biochemie > Zentrum für Biochemie Faculty of Medicine > Chirurgie > Abteilung Unfallchirurgie (Klinik und Poliklinik für Orthopädie und Unfallchirurgie) Faculty of Medicine > Dermatologie > Klinik und Poliklinik für Dermatologie und Venerologie Faculty of Medicine > Kinder- und Jugendmedizin > Klinik und Poliklinik für Kinder- und Jugendmedizin Zentrum für Molekulare Medizin
Subjects:	Medical sciences Medicine
Uncontrolled Keywords:	Keywords Language Human skin ; Dermal matrix ; Elastic fibers ; Oxytalan fibers ; Basement membrane ; Extracellular matrix proteins ; UV exposure ; Skin fibrosis ; Connective tissue disease English
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/81609