Kaur, Gurpinder, Stallmann, Daniela, Schanze, Nancy ORCID: 0000-0002-4012-103X, Laumann, Rosmarie, Heger, Lukas Andreas, Steinfurt, Johannes, Stachon, Peter, Peter, Karlheinz, Bode, Christoph, Moser, Martin, Ahrens, Ingo, Duerschmied, Daniel and Hortmann, Marcus (2019). Extracellular HtrA2 Induces Apoptosis in Human Umbilical Vein Endothelial Cells. Int. J. Mol. Sci., 20 (21). BASEL: MDPI. ISSN 1422-0067

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Abstract

The serine protease high-temperature-required protein A2 (HtrA2) has been identified as a key intracellular molecule promoting apoptosis in cells during ischemia reperfusion (IR) injury. IR injury in ST-segment elevation myocardial infarction (STEMI) contributes to overall myocardial damage. HtrA2 has further been shown to be significantly increased in the serum of patients with STEMI. In the present pilot study, we use human umbilical vein endothelial cells (HUVECs) to investigate whether extracellular HtrA2 induces apoptosis using Annexin V staining. Furthermore, we examine whether HtrA2 is released extracellularly after staurosporine-induced apoptosis using ELISA. We find that HtrA2 is released upon induction of apoptosis by staurosporine into the cell culture medium. Furthermore, treatment of HUVECs with extracellular HtrA2-induces apoptosis, while the addition of anti-HtrA2 antibodies reduces both HtrA2- and staurosporine-induced endothelial cell apoptosis. In conclusion, we show here that extracellular HtrA2 induces apoptosis in human endothelial cells, although the exact molecular mechanisms have to be investigated in future.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kaur, GurpinderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stallmann, DanielaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schanze, NancyUNSPECIFIEDorcid.org/0000-0002-4012-103XUNSPECIFIED
Laumann, RosmarieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Heger, Lukas AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Steinfurt, JohannesUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stachon, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Peter, KarlheinzUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bode, ChristophUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Moser, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ahrens, IngoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Duerschmied, DanielUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hortmann, MarcusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-128992
DOI: 10.3390/ijms20215446
Journal or Publication Title: Int. J. Mol. Sci.
Volume: 20
Number: 21
Date: 2019
Publisher: MDPI
Place of Publication: BASEL
ISSN: 1422-0067
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ISCHEMIA-REPERFUSION INJURY; ACUTE MYOCARDIAL-INFARCTION; ST-SEGMENT ELEVATION; CYTOCHROME-C; ISCHEMIA/REPERFUSION INJURY; DEATH; RELEASE; HEART; MECHANISMS; OMI/HTRA2Multiple languages
Biochemistry & Molecular Biology; Chemistry, MultidisciplinaryMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/12899

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