Universität zu Köln

Minges, Alexander ORCID: 0000-0001-7760-2753, Janssen, Dominik, Offermann, Sascha and Groth, Georg (2019). Efficient In Vivo Screening Method for the Identification of C-4 Photosynthesis Inhibitors Based on Cell Suspensions of the Single-Cell C-4 Plant Bienertia sinuspersici. Front. Plant Sci., 10. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-462X

Full text not available from this repository.

Abstract

The identification of novel herbicides is of crucial importance to modern agriculture. We developed an efficient in vivo assay based on oxygen evolution measurements using suspensions of chlorenchyma cells isolated from the single-cell C-4 plant Bienertia sinuspersici to identify and characterize inhibitors of C4 photosynthesis. This novel approach fills the gap between conventional in vitro assays for inhibitors targeting C-4 key enzymes and in vivo experiments on whole plants. The assay addresses inhibition of the target enzymes in a plant context thereby taking care of any reduced target inhibition due to metabolization or inadequate uptake of small molecule inhibitors across plant cell walls and membranes. Known small molecule inhibitors targeting C-4 photosynthesis were used to validate the approach. To this end, we tested pyruvate phosphate dikinase inhibitor bisindolylmaleimide IV and phosphoenolpyruvate carboxylase inhibitor okanin. Both inhibitors show inhibition of plant photosynthesis at half-maximal inhibitory concentrations in the sub-mM range and confirm their potential to act as a new class of C-4 selective inhibitors.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Minges, Alexander UNSPECIFIED orcid.org/0000-0001-7760-2753 UNSPECIFIED Janssen, Dominik UNSPECIFIED UNSPECIFIED UNSPECIFIED Offermann, Sascha UNSPECIFIED UNSPECIFIED UNSPECIFIED Groth, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-130105
DOI:	10.3389/fpls.2019.01350
Journal or Publication Title:	Front. Plant Sci.
Volume:	10
Date:	2019
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-462X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language C4 PHOSPHOENOLPYRUVATE CARBOXYLASE; KRANZ ANATOMY; EVOLUTION; CHENOPODIACEAE; DIKINASE; ENZYME; OPTIMIZATION; EXPRESSION; DISCOVERY; BIOASSAYS Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13010