Universität zu Köln

Lehmann, Sabrina, Sczyslo, Alissa, Froch-Cortis, Judith, Rothschild, Markus Alexander, Thevis, Mario, Andresen-Streichert, Hilke and Mercer-Chalmers-Bender, Katja ORCID: 0000-0002-3843-9065 (2019). Organ distribution of diclazepam, pyrazolam and 3-fluorophenmetrazine. Forensic Sci.Int., 303. CLARE: ELSEVIER IRELAND LTD. ISSN 1872-6283

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Abstract

The organ distribution of 3-fluorophenmetrazine (3-FPM), pyrazolam, diclazepam as well as its main metabolites delorazepam, lormetazepam and lorazepam, was investigated. A solid phase extraction (SPE) and a QuEChERS (acronym for quick, easy, cheap, effective, rugged and safe) - approach were used for the extraction of the analytes from human tissues, body fluids and stomach contents. The detection was performed on a liquid chromatography-tandem mass spectrometry system (LCMS/MS). The analytes of interest were detected in all body fluids and tissues. Results showed femoral blood concentrations of 10 mu g/L for 3-FPM, 28 mu g/L for pyrazolam, 1 mu g/L for diclazepam, 100 mu g/L for delorazepam, 6 mu g/L for lormetazepam, and 22 mu g/L for lorazepam. Tissues (muscle, kidney and liver) and bile exhibited higher concentrations of the mentioned analytes than in blood. Additional positive findings in femoral blood were for 2-fluoroamphetamine (2-FA, approx. 89 mu g/L), 2-flourometamphetamine (2-FMA, hint), methiopropamine (approx. 2.2 mu g/L), amphetamine (approx. 21 mu g/L) and caffeine (positive). Delorazepam showed the highest ratio of heart (C) and femoral blood (P) concentration (C/P ratio = 2.5), supported by the concentrations detected in psoas muscle (430 mu g/kg) and stomach content (approx. 210 mu g/L, absolute 84 mu g). The C/P ratio indicates that delorazepam displays susceptibility for postmortem redistribution (PMR), supported by the findings in muscle tissue. 3-FPM, pyrazolam, diclazepam, lorazepam and lormetazepam did apparently not exhibit any PMR. The cause of death, in conjunction with autopsy findings was concluded as a positional asphyxia promoted by poly-drug intoxication by arising from designer benzodiazepines and the presence of synthetic stimulants. (C) 2019 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lehmann, Sabrina UNSPECIFIED UNSPECIFIED UNSPECIFIED Sczyslo, Alissa UNSPECIFIED UNSPECIFIED UNSPECIFIED Froch-Cortis, Judith UNSPECIFIED UNSPECIFIED UNSPECIFIED Rothschild, Markus Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED Andresen-Streichert, Hilke UNSPECIFIED UNSPECIFIED UNSPECIFIED Mercer-Chalmers-Bender, Katja UNSPECIFIED orcid.org/0000-0002-3843-9065 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-131895
DOI:	10.1016/j.forsciint.2019.109959
Journal or Publication Title:	Forensic Sci.Int.
Volume:	303
Date:	2019
Publisher:	ELSEVIER IRELAND LTD
Place of Publication:	CLARE
ISSN:	1872-6283
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POSTMORTEM REDISTRIBUTION; PSYCHOACTIVE SUBSTANCES; LIQUID-CHROMATOGRAPHY; BENZODIAZEPINE USE; BLOOD-SAMPLES; QUECHERS; METABOLITES; ETIZOLAM; DRUGS; SERUM Multiple languages Medicine, Legal Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13189