Bovdilova, Anastasiia, Alexandre, Bruno M., Hoeppner, Astrid, Luis, Ines Matias ORCID: 0000-0001-8925-8857, Alvarez, Clarisa E., Bickel, David, Gohlke, Holger, Decker, Christina, Nagel-Steger, Luitgard ORCID: 0000-0001-5232-2149, Alseekh, Saleh ORCID: 0000-0003-2067-5235, Fernie, Alisdair R., Drincovich, Maria F., Abreu, Isabel A. and Maurino, Veronica G. (2019). Posttranslational Modification of the NADP-Malic Enzyme Involved in C-4 Photosynthesis Modulates the Enzymatic Activity during the Day. Plant Cell, 31 (10). S. 2525 - 2540. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-298X

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Abstract

Evolution of the C-4 photosynthetic pathway involved in some cases recruitment of housekeeping proteins through gene duplication and their further neofunctionalization. NADP-malic enzyme (ME), the most widespread C4 decarboxylase, has increased its catalytic efficiency and acquired regulatory properties that allowed it to participate in the C-4 pathway. Here, we show that regulation of maize (Zea mays) C-4-NADP-ME activity is much more elaborate than previously thought. Using mass spectrometry, we identified phosphorylation of the Ser419 residue of C-4-NADP-ME in protein extracts of maize leaves. The phosphorylation event increases in the light, with a peak at Zeitgeber time 2. Phosphorylation of ZmC(4)-NADP-ME drastically decreases its activity as shown by the low residual activity of the recombinant phosphomimetic mutant. Analysis of the crystal structure of C-4-NADP-ME indicated that Ser419 is involved in the binding of NADP at the active site. Molecular dynamics simulations and effective binding energy computations indicate a less favorable binding of the cofactor NADP in the phosphomimetic and the phosphorylated variants. We propose that phosphorylation of ZmC(4)-NADP-ME at Ser419 during the first hours in the light is a cellular mechanism that fine tunes the enzymatic activity to coordinate the carbon concentration mechanism with the CO2 fixation rate, probably to avoid CO2 leakiness from bundle sheath cells.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Bovdilova, AnastasiiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Alexandre, Bruno M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoeppner, AstridUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Luis, Ines MatiasUNSPECIFIEDorcid.org/0000-0001-8925-8857UNSPECIFIED
Alvarez, Clarisa E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bickel, DavidUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gohlke, HolgerUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Decker, ChristinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nagel-Steger, LuitgardUNSPECIFIEDorcid.org/0000-0001-5232-2149UNSPECIFIED
Alseekh, SalehUNSPECIFIEDorcid.org/0000-0003-2067-5235UNSPECIFIED
Fernie, Alisdair R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Drincovich, Maria F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Abreu, Isabel A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Maurino, Veronica G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-132746
DOI: 10.1105/tpc.19.00406
Journal or Publication Title: Plant Cell
Volume: 31
Number: 10
Page Range: S. 2525 - 2540
Date: 2019
Publisher: AMER SOC PLANT BIOLOGISTS
Place of Publication: ROCKVILLE
ISSN: 1532-298X
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
PROTEIN SECONDARY STRUCTURE; CRASSULACEAN ACID METABOLISM; MAIZE C(4)NADP-MALIC ENZYME; PHOSPHOENOLPYRUVATE CARBOXYLASE; MOLECULAR-DYNAMICS; ORTHOPHOSPHATE DIKINASE; REGULATORY PROTEINS; ATOMIC CHARGES; PHOSPHORYLATION; PYRUVATEMultiple languages
Biochemistry & Molecular Biology; Plant Sciences; Cell BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/13274

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