Universität zu Köln

Oswald, Evelyn, Kirschberg, Matthias ORCID: 0000-0002-3682-7371, Aubin, Francois, Alonso, Angel, Hufbauer, Martin, Akguel, Baki and Auvinen, Eeva ORCID: 0000-0002-2094-985X (2019). BetaHPV E6 and E7 colocalize with NuMa in dividing keratinocytes. Virus Genes, 55 (5). S. 600 - 610. DORDRECHT: SPRINGER. ISSN 1572-994X

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Abstract

Human papillomaviruses (HPVs) of genus betapapillomavirus (betaHPV) are implicated in skin carcinogenesis, but their exact role in keratinocyte transformation is poorly understood. We show an interaction of HPV5 and HPV8 oncoproteins E6 and E7 with the nuclear mitotic apparatus protein 1 (NuMA). Binding of E6 or E7 to NuMA induces little aneuploidy, cell cycle alterations, or aberrant centrosomes. Intracellular localization of NuMA is not altered by E6 and E7 expression in 2D cultures. However, the localization profile is predominantly cytoplasmic in 3D organotypic skin models. Both viral proteins colocalize with NuMA in interphase cells, while only E7 colocalizes with NuMA in mitotic cells. Intriguingly, a small subset of cells shows E7 at only one spindle pole, whereas NuMA is present at both poles. This dissimilar distribution of E7 at the spindle poles may alter cell differentiation, which may in turn be relevant for betaHPV-induced skin carcinogenesis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Oswald, Evelyn UNSPECIFIED UNSPECIFIED UNSPECIFIED Kirschberg, Matthias UNSPECIFIED orcid.org/0000-0002-3682-7371 UNSPECIFIED Aubin, Francois UNSPECIFIED UNSPECIFIED UNSPECIFIED Alonso, Angel UNSPECIFIED UNSPECIFIED UNSPECIFIED Hufbauer, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Akguel, Baki UNSPECIFIED UNSPECIFIED UNSPECIFIED Auvinen, Eeva UNSPECIFIED orcid.org/0000-0002-2094-985X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-133291
DOI:	10.1007/s11262-019-01685-9
Journal or Publication Title:	Virus Genes
Volume:	55
Number:	5
Page Range:	S. 600 - 610
Date:	2019
Publisher:	SPRINGER
Place of Publication:	DORDRECHT
ISSN:	1572-994X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN-PAPILLOMAVIRUS TYPE-16; EARLY GENES; PROTEIN; EXPRESSION; DIFFERENTIATION; CHROMOSOME; ORGANIZATION; CENTROSOME; ABROGATE; INVASION Multiple languages Genetics & Heredity; Virology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13329