Universität zu Köln

Koprivova, Anna, Schuck, Stefan ORCID: 0000-0002-3959-4884, Jacoby, Richard P., Klinkhammer, Irene, Welter, Bastian, Leson, Lisa, Martyn, Anna, Nauen, Julia, Grabenhorst, Niklas, Mandelkow, Jan F., Zuccaro, Alga ORCID: 0000-0002-8026-0114, Zeier, Juergen and Kopriva, Stanislav ORCID: 0000-0002-7416-6551 (2019). Root-specific camalexin biosynthesis controls the plant growth-promoting effects of multiple bacterial strains. Proc. Natl. Acad. Sci. U. S. A., 116 (31). S. 15735 - 15745. WASHINGTON: NATL ACAD SCIENCES. ISSN 0027-8424

Full text not available from this repository.

Abstract

Plants in their natural ecosystems interact with numerous microorganisms, but how they influence their microbiota is still elusive. We observed that sulfatase activity in soil, which can be used as a measure of rhizosphere microbial activity, is differently affected by Arabidopsis accessions. Following a genome-wide association analysis of the variation in sulfatase activity we identified a candidate gene encoding an uncharacterized cytochrome P450, CYP71A27. Loss of this gene resulted in 2 different and independent microbiota-specific phenotypes: A lower sulfatase activity in the rhizosphere and a loss of plant growth-promoting effect by Pseudomonas sp. CH267. On the other hand, tolerance to leaf pathogens was not affected, which agreed with prevalent expression of CYP71A27 in the root vasculature. The phenotypes of cyp71A27 mutant were similar to those of cyp71A12 and cyp71A13, known mutants in synthesis of camalexin, a sulfur-containing indolic defense compound. Indeed, the cyp71A27 mutant accumulated less camalexin in the roots upon elicitation with silver nitrate or flagellin. Importantly, addition of camalexin complemented both the sulfatase activity and the loss of plant growth promotion by Pseudomonas sp. CH267. Two alleles of CYP71A27 were identified among Arabidopsis accessions, differing by a substitution of Glu373 by Gln, which correlated with the ability to induce camalexin synthesis and to gain fresh weight in response to Pseudomonas sp. CH267. Thus, CYP71A27 is an additional component in the camalexin synthesis pathway, contributing specifically to the control of plant microbe interactions in the root.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Koprivova, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Schuck, Stefan UNSPECIFIED orcid.org/0000-0002-3959-4884 UNSPECIFIED Jacoby, Richard P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Klinkhammer, Irene UNSPECIFIED UNSPECIFIED UNSPECIFIED Welter, Bastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Leson, Lisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Martyn, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Nauen, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Grabenhorst, Niklas UNSPECIFIED UNSPECIFIED UNSPECIFIED Mandelkow, Jan F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zuccaro, Alga UNSPECIFIED orcid.org/0000-0002-8026-0114 UNSPECIFIED Zeier, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Kopriva, Stanislav UNSPECIFIED orcid.org/0000-0002-7416-6551 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-134538
DOI:	10.1073/pnas.1818604116
Journal or Publication Title:	Proc. Natl. Acad. Sci. U. S. A.
Volume:	116
Number:	31
Page Range:	S. 15735 - 15745
Date:	2019
Publisher:	NATL ACAD SCIENCES
Place of Publication:	WASHINGTON
ISSN:	0027-8424
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ARABIDOPSIS-THALIANA; SECONDARY METABOLITES; NATURAL VARIATION; SALICYLIC-ACID; RESISTANCE; MICROBIOTA; COLONIZATION; SYMBIOSIS; RESPONSES; REQUIRES Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13453