Universität zu Köln

Goh, Falicia Qi Yun, Jeyakani, Justin, Tipthara, Phornpimon, Cazenave-Gassiot, Amaury ORCID: 0000-0002-3050-634X, Ghosh, Rajoshi, Bogard, Nicholas, Yeo, Zhenxuan, Wong, Gane Ka-Shu, Melkonian, Michael, Wenk, Markus R. and Clarke, Neil D. (2019). Gains and losses of metabolic function inferred from a phylotranscriptomic analysis of algae. Sci Rep, 9. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Hidden Markov models representing 167 protein sequence families were used to infer the presence or absence of homologs within the transcriptomes of 183 algal species/strains. Statistical analyses of the distribution of HMM hits across major clades of algae, or at branch points on the phylogenetic tree of 98 chlorophytes, confirmed and extended known cases of metabolic loss and gain, most notably the loss of the mevalonate pathway for terpenoid synthesis in green algae but not, as we show here, in the streptophyte algae. Evidence for novel events was found as well, most remarkably in the recurrent and coordinated gain or loss of enzymes for the glyoxylate shunt. We find, as well, a curious pattern of retention (or re-gain) of HMG-CoA synthase in chlorophytes that have otherwise lost the mevalonate pathway, suggesting a novel, co-opted function for this enzyme in select lineages. Finally, we find striking, phylogenetically linked distributions of coding sequences for three pathways that synthesize the major membrane lipid phosphatidylcholine, and a complementary phylogenetic distribution pattern for the non-phospholipid DGTS (diacyl-glyceryl-trimethylhomoserine). Mass spectrometric analysis of lipids from 25 species was used to validate the inference of DGTS synthesis from sequence data.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Goh, Falicia Qi Yun UNSPECIFIED UNSPECIFIED UNSPECIFIED Jeyakani, Justin UNSPECIFIED UNSPECIFIED UNSPECIFIED Tipthara, Phornpimon UNSPECIFIED UNSPECIFIED UNSPECIFIED Cazenave-Gassiot, Amaury UNSPECIFIED orcid.org/0000-0002-3050-634X UNSPECIFIED Ghosh, Rajoshi UNSPECIFIED UNSPECIFIED UNSPECIFIED Bogard, Nicholas UNSPECIFIED UNSPECIFIED UNSPECIFIED Yeo, Zhenxuan UNSPECIFIED UNSPECIFIED UNSPECIFIED Wong, Gane Ka-Shu UNSPECIFIED UNSPECIFIED UNSPECIFIED Melkonian, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Wenk, Markus R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Clarke, Neil D. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-134968
DOI:	10.1038/s41598-019-46869-3
Journal or Publication Title:	Sci Rep
Volume:	9
Date:	2019
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ISOPRENOID BIOSYNTHESIS; EVOLUTION; MEVALONATE; PATHWAYS; ORIGINS Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13496