Universität zu Köln

Villajuana-Bonequi, Mitzi, Matei, Alexandra, Ernst, Corinna, Hallab, Asis, Usadel, Bjoern and Doehlemann, Gunther ORCID: 0000-0002-7353-8456 (2019). Cell type specific transcriptional reprogramming of maize leaves during Ustilago maydis induced tumor formation. Sci Rep, 9. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Ustilago maydis is a biotrophic pathogen and well-established genetic model to understand the molecular basis of biotrophic interactions. U. maydis suppresses plant defense and induces tumors on all aerial parts of its host plant maize. In a previous study we found that U. maydis induced leaf tumor formation builds on two major processes: the induction of hypertrophy in the mesophyll and the induction of cell division (hyperplasia) in the bundle sheath. In this study we analyzed the cell-type specific transcriptome of maize leaves 4 days post infection. This analysis allowed identification of key features underlying the hypertrophic and hyperplasic cell identities derived from mesophyll and bundle sheath cells, respectively. We examined the differentially expressed (DE) genes with particular focus on maize cell cycle genes and found that three A-type cyclins, one B-, D- and T-type are upregulated in the hyperplasic tumorous cells, in which the U. maydis effector protein See1 promotes cell division. Additionally, most of the proteins involved in the formation of the pre-replication complex (pre-RC, that assure that each daughter cell receives identic DNA copies), the transcription factors E2F and DPa as well as several D-type cyclins are deregulated in the hypertrophic cells.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Villajuana-Bonequi, Mitzi UNSPECIFIED UNSPECIFIED UNSPECIFIED Matei, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Ernst, Corinna UNSPECIFIED UNSPECIFIED UNSPECIFIED Hallab, Asis UNSPECIFIED UNSPECIFIED UNSPECIFIED Usadel, Bjoern UNSPECIFIED UNSPECIFIED UNSPECIFIED Doehlemann, Gunther UNSPECIFIED orcid.org/0000-0002-7353-8456 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-135122
DOI:	10.1038/s41598-019-46734-3
Journal or Publication Title:	Sci Rep
Volume:	9
Date:	2019
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GENOME-WIDE ANALYSIS; CYCLE PROGRESSION; GENE-EXPRESSION; DNA-REPLICATION; HIGHER-PLANTS; LEAF GROWTH; PROTEIN; FAMILY; PROLIFERATION; SUMOYLATION Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13512