Universität zu Köln

Chaigne, Benjamin, Clary, Guilhem, Le Gall, Morgane, Dumoitier, Nicolas, Fernandez, Claire, Lofek, Sebastien, Chafey, Philippe, Moinzadeh, Pia, Krieg, Thomas, Denton, Christopher P. and Mouthon, Luc (2019). Proteomic Analysis of Human Scleroderma Fibroblasts Response to Transforming Growth Factor-ss. Proteom. Clin. Appl., 13 (4). WEINHEIM: WILEY-V C H VERLAG GMBH. ISSN 1862-8354

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Abstract

Purpose Systemic sclerosis (SSc) is characterized by autoimmunity, vasculopathy and fibrosis. Fibrosis is due to an activation of fibroblasts by the transforming growth factor-ss (TGF-ss). This study investigates the proteomic response of SSc fibroblasts to TGF-ss. Experimental design Skin fibroblasts from diffuse SSc patients and healthy controls (HC) are cultured with or without TGF-ss. Two-dimensional differential in-gel electrophoresis and mass spectrometry (MS) combined with Ingenuity Pathway analysis (IPA) and Panther/David software analyze proteins differentially expressed between groups. Real-time cell analyzer (RTCA) assesses fibroblast proliferation and viability. Results Two-hundred-and-seventy-nine proteins are differentially expressed between groups. Principal component analysis shows significant differences between groups. IPA shows specific process networks such as actin cytoskeleton and integrin signaling. Panther and David software show predominant biological processes such as cellular and metabolic processes. TGF-ss enhances protein synthesis and protein pathways. IPA and RTCA suggest the involvement of epidermal growth factor receptor (EGFR) and phosphatidylinositol 3 kinase (Pi3K). Conclusions and clinical relevance That the proteome of fibroblasts differs between SSc patients and HC is confirmed, and it is demonstrated that fibroblasts exacerbate their proteomic phenotype upon stimulation with TGF-ss. EGFR and Pi3K are highlighted as proteins of interest in SSc fibroblasts.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Chaigne, Benjamin UNSPECIFIED UNSPECIFIED UNSPECIFIED Clary, Guilhem UNSPECIFIED UNSPECIFIED UNSPECIFIED Le Gall, Morgane UNSPECIFIED UNSPECIFIED UNSPECIFIED Dumoitier, Nicolas UNSPECIFIED UNSPECIFIED UNSPECIFIED Fernandez, Claire UNSPECIFIED UNSPECIFIED UNSPECIFIED Lofek, Sebastien UNSPECIFIED UNSPECIFIED UNSPECIFIED Chafey, Philippe UNSPECIFIED UNSPECIFIED UNSPECIFIED Moinzadeh, Pia UNSPECIFIED UNSPECIFIED UNSPECIFIED Krieg, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Denton, Christopher P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mouthon, Luc UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-136096
DOI:	10.1002/prca.201800069
Journal or Publication Title:	Proteom. Clin. Appl.
Volume:	13
Number:	4
Date:	2019
Publisher:	WILEY-V C H VERLAG GMBH
Place of Publication:	WEINHEIM
ISSN:	1862-8354
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FACTOR RECEPTOR EGFR; SYSTEMIC-SCLEROSIS; FACTOR-BETA; DERMAL FIBROBLASTS; ELEVATED LEVELS; UP-REGULATION; EXPRESSION; AUTOANTIBODIES; IDENTIFICATION; PATHOGENESIS Multiple languages Biochemical Research Methods; Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13609