Farowski, Fedja, Solbach, Philipp, Tsakmaklis, Anastasia, Brodesser, Susanne, Aguilar, Rebeca Cruz, Cornely, Oliver A., Dettmer, Katja, Higgins, Paul G. ORCID: 0000-0001-8677-9454, Suerbaum, Sebastian, Jazmati, Nathalie, Oefner, Peter J. and Vehreschild, Maria J. G. T. (2019). Potential biomarkers to predict outcome of faecal microbiota transfer for recurrent Clostridioides difficile infection. Dig. Liver Dis., 51 (7). S. 944 - 952. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1878-3562

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Abstract

Background & Aims: Faecal microbiota transplantation (FMT) has proven high clinical efficacy in the management of recurrent Clostridioides difficile infection (rCDI) with cure rates of over 80% after a single treatment. Nevertheless, the reasons for failure in the remaining 20% remain elusive. The aim of the present study was to investigate different potential predictors of response to FMT. Methods: Faecal specimens of sixteen patients undergoing FMT for rCDI, as well as samples from the respective donors were collected and analyzed by 16S rRNA gene profiling, bile acid-inducible (baiCD) gene specific qPCR, and liquid chromatography tandem-mass spectrometry (LC-MS/MS) to quantify the concentrations of primary and secondary bile acids. Results: Using the faecal concentration of the secondary bile acid lithocholic acid (LCA) within the patient specimens, we were able to predict response to FMT (accuracy 95.2%, sensitivity 100%, specificity 90.9%). By combining the faecal LCA concentration with the urinary pCS concentration, an accuracy of 100% was achieved. Conclusion: LCA appears to be a promising marker candidate for prediction of clinical response to FMT. Other makers, such as urinary concentration of pCS, but not 3-IS, might be used to improve accuracy of prediction. Further studies are warranted to validate these candidate markers. (C) 2019 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Farowski, FedjaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Solbach, PhilippUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tsakmaklis, AnastasiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brodesser, SusanneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Aguilar, Rebeca CruzUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cornely, Oliver A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dettmer, KatjaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Higgins, Paul G.UNSPECIFIEDorcid.org/0000-0001-8677-9454UNSPECIFIED
Suerbaum, SebastianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jazmati, NathalieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Oefner, Peter J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vehreschild, Maria J. G. T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-136869
DOI: 10.1016/j.dld.2019.01.012
Journal or Publication Title: Dig. Liver Dis.
Volume: 51
Number: 7
Page Range: S. 944 - 952
Date: 2019
Publisher: ELSEVIER SCIENCE INC
Place of Publication: NEW YORK
ISSN: 1878-3562
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
SPORE GERMINATION; ACID; BILE; TRANSPLANTATION; ANTIBIOTICS; FIDAXOMICIN; VANCOMYCIN; BURDENMultiple languages
Gastroenterology & HepatologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/13686

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