Universität zu Köln

Chopra, Divykriti, Mapar, Mona, Stephan, Lisa ORCID: 0000-0002-1296-7548, Albani, Maria C., Deneer, Anna, Coupland, George ORCID: 0000-0001-6988-4172, Willing, Eva-Maria, Schellmann, Swen, Schneeberger, Korbinian, Fleck, Christian, Schrader, Andrea ORCID: 0000-0002-3879-7057 and Huelskamp, Martin (2019). Genetic and molecular analysis of trichome development in Arabis alpina. Proc. Natl. Acad. Sci. U. S. A., 116 (24). S. 12078 - 12084. WASHINGTON: NATL ACAD SCIENCES. ISSN 0027-8424

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Abstract

The genetic and molecular analysis of trichome development in Arabidopsis thaliana has generated a detailed knowledge about the underlying regulatory genes and networks. However, how rapidly these mechanisms diverge during evolution is unknown. To address this problem, we used an unbiased forward genetic approach to identify most genes involved in trichome development in the related crucifer species Arabis alpina. In general, we found most trichome mutant classes known in A. thaliana. We identified orthologous genes of the relevant A. thaliana genes by sequence similarity and synteny and sequenced candidate genes in the A. alpina mutants. While in most cases we found a highly similar genephenotype relationship as known from Arabidopsis, there were also striking differences in the regulation of trichome patterning, differentiation, and morphogenesis. Our analysis of trichome patterning suggests that the formation of two classes of trichomes is regulated differentially by the homeodomain transcription factor AaGL2. Moreover, we show that overexpression of the GL3 basic helix-loop-helix transcription factor in A. alpina leads to the opposite phenotype as described in A. thaliana. Mathematical modeling helps to explain how this nonintuitive behavior can be explained by different ratios of GL3 and GL1 in the two species.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Chopra, Divykriti UNSPECIFIED UNSPECIFIED UNSPECIFIED Mapar, Mona UNSPECIFIED UNSPECIFIED UNSPECIFIED Stephan, Lisa UNSPECIFIED orcid.org/0000-0002-1296-7548 UNSPECIFIED Albani, Maria C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Deneer, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Coupland, George UNSPECIFIED orcid.org/0000-0001-6988-4172 UNSPECIFIED Willing, Eva-Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Schellmann, Swen UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneeberger, Korbinian UNSPECIFIED UNSPECIFIED UNSPECIFIED Fleck, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Schrader, Andrea UNSPECIFIED orcid.org/0000-0002-3879-7057 UNSPECIFIED Huelskamp, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-137926
DOI:	10.1073/pnas.1819440116
Journal or Publication Title:	Proc. Natl. Acad. Sci. U. S. A.
Volume:	116
Number:	24
Page Range:	S. 12078 - 12084
Date:	2019
Publisher:	NATL ACAD SCIENCES
Place of Publication:	WASHINGTON
ISSN:	0027-8424
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ARABIDOPSIS; PROTEIN; EVOLUTION; DATABASE; NETWORK; ENCODES; COMPLEX; MUTANT; HAIRS; GL3 Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13792