Universität zu Köln

Mylonas, Constantine and Tessarz, Peter (2019). NET-prism enables RNA polymerase-dedicated transcriptional interrogation at nucleotide resolution. RNA Biol., 16 (9). S. 1156 - 1166. PHILADELPHIA: TAYLOR & FRANCIS INC. ISSN 1555-8584

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Abstract

The advent of quantitative approaches that enable interrogation of transcription at single nucleotide resolution has allowed a novel understanding of transcriptional regulation previously undefined. However, little is known, at such high resolution, how transcription factors directly influence RNA Pol II pausing and directionality. To map the impact of transcription/elongation factors on transcription dynamics genome-wide at base pair resolution, we developed an adapted NET-seq protocol called NET-prism (Native Elongating Transcription by Polymerase-Regulated Immunoprecipitants in the Mammalian genome). Application of NET-prism on elongation factors (Spt6, Ssrp1), splicing factors (Sf1), and components of the pre-initiation complex (PIC) (TFIID, and Mediator) reveals their inherent command on transcription dynamics, with regards to directionality and pausing over promoters, splice sites, and enhancers/super-enhancers. NET-prism will be broadly applicable as it exposes transcription factor/Pol II dependent topographic specificity and thus, a new degree of regulatory complexity during gene expression.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mylonas, Constantine UNSPECIFIED UNSPECIFIED UNSPECIFIED Tessarz, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-138267
DOI:	10.1080/15476286.2019.1621625
Journal or Publication Title:	RNA Biol.
Volume:	16
Number:	9
Page Range:	S. 1156 - 1166
Date:	2019
Publisher:	TAYLOR & FRANCIS INC
Place of Publication:	PHILADELPHIA
ISSN:	1555-8584
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SUPER-ENHANCERS; FACTOR-BINDING; ELONGATION; PROTEOME; EXTRACTION; INITIATION; PROMOTERS; REGIONS Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13826