Universität zu Köln

Schildgen, Oliver ORCID: 0000-0003-4297-9627, Graeper, Sascha, Bluemel, Johannes, Kuelshammer, Manuel and Matz, Bertfried (2019). Temperature-sensitive origin-binding protein as a tool for investigations of herpes simplex virus activities in vivo. J. Gen. Virol., 100 (1). S. 105 - 118. LONDON: MICROBIOLOGY SOC. ISSN 1465-2099

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Abstract

While it is fairly clear that herpes simplex virus (HSV) DNA replication requires at least seven virus-encoded proteins in concert with various host cell factors, the mode of this process in infected cells is still poorly understood. Using HSV-1 mutants bearing temperature-sensitive (ts) lesions in the UL9 gene, we previously found that the origin-binding protein (OBP), a product of the UL9 gene, is only needed in the first 6 hours post-infection. As this finding was just a simple support for the hypothesis of a biphasic replication mode, we became convinced through these earlier studies that the mutants tsR and tsS might represent suitable tools for more accurate investigations in vivo. However, prior to engaging in highly sophisticated research projects, knowledge of the biochemical features of the mutated versions of OBP appeared to be essential. The results of our present study demonstrate that (i) tsR is most appropriate for cell biological studies, where only immediate early and early HSV gene products are being expressed without the concomital viral DNA replication, and (ii) tsS is a prime candidate for the analysis of HSV DNA replication processes because of its reversibly thermosensitive OBPATPase, which allows one to switch on the initiation of DNA synthesis precisely.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schildgen, Oliver UNSPECIFIED orcid.org/0000-0003-4297-9627 UNSPECIFIED Graeper, Sascha UNSPECIFIED UNSPECIFIED UNSPECIFIED Bluemel, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuelshammer, Manuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Matz, Bertfried UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-140503
DOI:	10.1099/jgv.0.001184
Journal or Publication Title:	J. Gen. Virol.
Volume:	100
Number:	1
Page Range:	S. 105 - 118
Date:	2019
Publisher:	MICROBIOLOGY SOC
Place of Publication:	LONDON
ISSN:	1465-2099
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CONSERVED HELICASE MOTIFS; DNA-REPLICATION; GENE-FUNCTION; TYPE-1; UL9; GENOME; AMPLIFICATION; MUTATIONS; REGION; INTEGRATION Multiple languages Biotechnology & Applied Microbiology; Virology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14050