Universität zu Köln

Frazao, Nelson ORCID: 0000-0003-3509-8338, Sousa, Ana, Laessig, Michael and Gordo, Isabel ORCID: 0000-0003-1622-5143 (2019). Horizontal gene transfer overrides mutation in Escherichia coli colonizing the mammalian gut. Proc. Natl. Acad. Sci. U. S. A., 116 (36). S. 17906 - 17916. WASHINGTON: NATL ACAD SCIENCES. ISSN 0027-8424

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Abstract

Bacteria evolve by mutation accumulation in laboratory experiments, but tempo and mode of evolution in natural environments are largely unknown. Here, we study the ubiquitous natural process of host colonization by commensal bacteria. We show, by experimental evolution of Escherichia coli in the mouse intestine, that the ecology of the gut controls the pace and mode of evolution of a new invading bacterial strain. If a resident E. coli strain is present in the gut, the invading strain evolves by rapid horizontal gene transfer (HGT), which precedes and outweighs evolution by accumulation of mutations. HGT is driven by 2 bacteriophages carried by the resident strain, which cause an epidemic phage infection of the invader. These dynamics are followed by subsequent evolution by clonal interference of genetically diverse lineages of phage-carrying (lysogenic) bacteria. We show that the genes uptaken by HGT enhance the metabolism of specific gut carbon sources and provide a fitness advantage to lysogenic invader lineages. A minimal dynamical model explains the temporal pattern of phage epidemics and the complex evolutionary outcome of phage-mediated selection. We conclude that phage-driven HGT is a key eco-evolutionary driving force of gut colonization-it accelerates evolution and promotes genetic diversity of commensal bacteria.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Frazao, Nelson UNSPECIFIED orcid.org/0000-0003-3509-8338 UNSPECIFIED Sousa, Ana UNSPECIFIED UNSPECIFIED UNSPECIFIED Laessig, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Gordo, Isabel UNSPECIFIED orcid.org/0000-0003-1622-5143 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-141786
DOI:	10.1073/pnas.1906958116
Journal or Publication Title:	Proc. Natl. Acad. Sci. U. S. A.
Volume:	116
Number:	36
Page Range:	S. 17906 - 17916
Date:	2019
Publisher:	NATL ACAD SCIENCES
Place of Publication:	WASHINGTON
ISSN:	0027-8424
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Physics > Institut für Biologische Physik
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXPERIMENTAL EVOLUTION; GENOME EVOLUTION; PHAGE; HOST; DYNAMICS; BACTERIA; ADAPTATION; MICROBIOTA; FITNESS; K-12 Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14178