Universität zu Köln

Saez, Isabel, Gerbracht, Jennifer, V, Koyuncu, Seda, Lee, Hyun Ju, Horn, Moritz, Kroef, Virginia, Denzel, Martin S., Dieterich, Christoph, Gehring, Niels H. ORCID: 0000-0001-7792-1164 and Vilchez, David ORCID: 0000-0002-0801-0743 (2020). The E3 ubiquitin ligase UBR5 interacts with the H/ACA ribonucleoprotein complex and regulates ribosomal RNA biogenesis in embryonic stem cells. FEBS Lett., 594 (1). S. 175 - 189. HOBOKEN: WILEY. ISSN 1873-3468

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Abstract

UBR5 is an E3 ubiquitin ligase involved in distinct processes such as transcriptional regulation and development. UBR5 is highly upregulated in embryonic stem cells (ESCs), whereas its expression decreases with differentiation, suggesting a role for UBR5 in ESC function. However, little is known about how UBR5 regulates ESC identity. Here, we define the protein interactome of UBR5 in ESCs and find interactions with distinct components of the H/ACA ribonucleoprotein complex, which is required for proper maturation of ribosomal RNA (rRNA). Notably, loss of UBR5 induces an abnormal accumulation of rRNA processing intermediates, resulting in diminished ribosomal levels. Consequently, lack of UBR5 triggers an increase in p53 levels and a concomitant decrease in cellular proliferation rates. Thus, our results indicate a link between UBR5 and rRNA maturation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Saez, Isabel UNSPECIFIED UNSPECIFIED UNSPECIFIED Gerbracht, Jennifer, V UNSPECIFIED UNSPECIFIED UNSPECIFIED Koyuncu, Seda UNSPECIFIED UNSPECIFIED UNSPECIFIED Lee, Hyun Ju UNSPECIFIED UNSPECIFIED UNSPECIFIED Horn, Moritz UNSPECIFIED UNSPECIFIED UNSPECIFIED Kroef, Virginia UNSPECIFIED UNSPECIFIED UNSPECIFIED Denzel, Martin S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dieterich, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Gehring, Niels H. UNSPECIFIED orcid.org/0000-0001-7792-1164 UNSPECIFIED Vilchez, David UNSPECIFIED orcid.org/0000-0002-0801-0743 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-144614
DOI:	10.1002/1873-3468.13559
Journal or Publication Title:	FEBS Lett.
Volume:	594
Number:	1
Page Range:	S. 175 - 189
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1873-3468
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HYPERPLASTIC DISCS PROTEIN; POLY(A)-BINDING PROTEIN; EDD; IDENTIFICATION; DOMAIN; RECEPTOR; BINDING; KINASE; GENE; ACTIVATION Multiple languages Biochemistry & Molecular Biology; Biophysics; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14461