Universität zu Köln

Ruehr, Peter T. and Lambertz, Markus ORCID: 0000-0001-8348-9347 (2019). Surface contrast enhancement of integumentary structures in X-ray tomography. J. Anat., 235 (2). S. 379 - 386. HOBOKEN: WILEY. ISSN 1469-7580

Full text not available from this repository.

Abstract

Micro-computed tomography (mu CT) has become standard in the biological sciences to reconstruct, display and analyse 3D models of all kinds of organisms. However, it is often impossible to capture fine details of the surface and the internal anatomy at the same time with sufficient contrast. Here we introduce a new approach for the selective contrast-enhancement of integumentary surface structures. The method relies on conventional and readily available sputter coaters to cover the entire sample with a thin layer of gold atoms. This approach proved successful on a diverse array of plants and animals. On average, we achieved a 14.48-fold gain of surface contrast (ranging from 2.42-fold to 86.93-fold) compared with untreated specimens. Even X-ray-transparent samples such as spider silk became accessible via mu CT. This selective contrast-enhancement, makes it possible to digitally reconstruct fine surface structures with low absorbance while the tissue-dependent grey value resolution of the inner anatomy is maintained and remains fully visualisable. The methodology is suited for a broad scientific application across biology and other sciences employing (mu)CT, as well as educative and public outreach purposes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ruehr, Peter T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lambertz, Markus UNSPECIFIED orcid.org/0000-0001-8348-9347 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-145151
DOI:	10.1111/joa.13008
Journal or Publication Title:	J. Anat.
Volume:	235
Number:	2
Page Range:	S. 379 - 386
Date:	2019
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1469-7580
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language COMPUTED-TOMOGRAPHY; HIGH-RESOLUTION; ANATOMY; CT Multiple languages Anatomy & Morphology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14515